The Relationship Between β2 Adrenergic Receptor Gene Polymorphisms And Essential Hypertension

Objective: To investigate whether the variants A46G and C79G of ?2-adrenergic receptor gene are involved in the pathogenesis of essential hypertension in Chinese Han population from the area of Hubei province.Methods: Genomic DNA was extracted from peripheral blood leukocytes by improved idoic sodium method in both essential hypertension group and normotensive group.the distribution of 3 2-AR 46A/G and 79C/G locus polymorphism was identified with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 123 unrelated health controls and 109 patients with essential hypertension. Automated Biochemical Analyzer was used to measure the plasma lipid level.Results: For the Argl6Gly polymorphism,the allelic frequencies were 0.64 for the A allele and 0.36 for the G allele in normotensive subjects,0.54 for the A allele and 0.46 for the G allele in essential hypertension patients. There was a significant increase in the frequency of G allele gene in hypertensive compared with health group (P<0.05).No significant diffence was observed in the genotypes distribution of AA, AG, GG at locus 16 of 2-AR gene between the EH group(0.42,0.45 , 0.13)and the NT group(0.28, 0.52, 0.20)(P>0.05).Essential hypertension subjects who posses the G allele had a significant higher diastolic blood pressure level than those lacking the G allele(P<0.05). For the Gln27Glu polymorphism ,the allelic frequencies were 0.09 for the G allele and 0.91 for the C allele in normotensive subjects,0.07 for the G allele and 0.93 for the C allele in essential hypertension patients. No distinct difference in genotypes distribution (P>0.05) and allelic frequencies (P>0.05) between essential hypertension and normotensive subjects.There was not significant difference in serum lipids except high density lipoprotein-cholesterol level.Conclusions: The results suggest that the polymorphism in 2-AR gene of46A/G may be involved in the pathogensis of essential hypertension in Chinese people, while the 79C/G variant may not play an important role in the etiology of hypertension in Chinese.There is a very strong linkage disequilibrium between the two single necleotide polymorphism(SNP),but there are not significant interaction between them.