| Background and Objective: Osteosarcoma is the most frequent primary maligant bone tumor with a peak incidence in the second and third decade of life and its biological behavior is always highly maligant.Its incidence lie in the second place in the primary maligant bone tumor.As the other tumors,the osteosarcomatous genesis is correlated with apoptosis mechanism. It is now widespread to think, occurrence or not of cell apoptosis is a coefficient result of various factors, but that regulation of gene is a link of the very key of mechanism of apoptosis.Survivin is a novel member of the inhibitor of apoptosis proteins (LAP) family, it can positionicularily expresses in the tumor tissue, the embryo and the growth fetus tissue, but not in the terminally differentiated adult tissues (thymus, genital gland excepted). Current studies suggest that survivin is implicated in both control of apoptosis and regultion of cell division. Survivin can block apoptosis by direct inhibition of activation of Caspase-3 or -7. Its overexpression is closely ralated with carcinogenesis and prognostic of tumor;PTEN, a novel tumor suppressor gene,also plays an important role in both control of cell growth and apoptosis.But its biological effect is reverse in contrast with survivin. It can positionicularily dephosphate phosphatidylinositol 3,4,5-trisphosphate(PIP3) in site D3, which can block the signaling conduction pathway of PIP3/Akt and induce cell death. P53 play a centre role in control of cell cycle. Its overexpression can promoteunlimited growth of cell and carcinogenesis.As for expression and regulation mechanism of above three kinds of genes in the osteosarcoma, current yet no be reported in our internal and external periodical. The purpose of this text is to study the value of these genes that occurrence, development, clinical diagnosis and prognostic distinguishing in osteosarcoma, and provide a new direction of full of foreground for gene therapy of osteosarcoma.Methods: In this research the immunohistochemistory S-P method was adopted for detection of expression of survivin, PTEN, p53 in osteosarcoma. Thirty specimens of human OS tissues(OST) were obtained. The control groups were 10 cases osteochondroma tissues(OCT).There was a significant difference when P<0.05.Results:1. Expression of survivin protein: 1.1 The positive immunostaining rate of survivin in OCT and OST were 0.0%, 83.3%, respectively. There was significant difference (P<0.05). Comparing the staining intensity, there was significant difference (P>0.05). 1.2 Expression of survivin protein in OST: In grade I of OST, the positive immunostaining rate of survivin was 37.5%, in which negative, weak, moderate and strong immunostaining wereS, 0, 2, 1, repectively; In grade II+III of OST, the positive immunostaining rate of survivin was 100%, in which negative, weak, moderate and strong immunostaining wereO, 7, 13, 2. Compare the staining rate and intensity, there were significant differinces (P<0.05).2,. Expression of PTEN protein: 2.1 In OCT, the positive immunostaining rate of PTEN was 80%, in which negative, weak, moderate and strong immunostaining were 2, 1, 1, 6, repectively; In OST, the positive immunostaining rate of PTEN was 73.3%, in which negative, weak, moderate and strong immunostaining were 8, 6, 13, 3, repectively. Comparing the staining rate, there was no significant difference (P>0.05). Comparing the staining intensity, there was significant difference (P>0.05). 2.2 Expression of PTEN in protein in OST: In grade I of OST, the positive immunostaining rate of survivin was 75%, inwhich negative, weak, moderate and strong immunostaining were 2, 2, 3, 1, repectively; In grade II+III of OST, the positive immunostaining rate of survivin was 68.2%, in which negative, weak, moderate and strong immunostaining were 6, 4, 10, 2. Compare the staining rate and intensity, there were no significant differirices (P>0.05).3. Expression of p53 protein: 3.1 The positivie immunostaining rates of p53 in OCT and OST were 20%, 63.3%. respectively. There was s...
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