Microsatellite Change And Its Relationship With TSGs In Bladdder Transitional Cell Carcinoma

Objective To study the expression of microsatellite change in bladddertransitional cell carcinoma(BTCC) and its relationship with PTEN,nm23-H1and KAI1. Methods Tumor samples from 40 patients with BTCC were analyzed formicrosatellite change and its relationship with PTEN,nm23-H1 and KAI1 byPCR. Results In 32 of 40 BTCC patients (80%) microsatellite instability(MSI)or loss of heterozygosity(LOH) were found on at least one microsatellite locus.The positive rate of D9S162, D11S554 and IFN-A1 was 55%(22/40),40%(16/40) and 50%(20/40) respectively, while the 20 non bladder cancerindividuals had no changes at any of these loci. The result was not associatedwith grade or stage of the tumor. There was statistical difference between thetwo groups. The positive rate of nm23-H1 was associated with stage of thetumor and that of PTEN. Conclusions Microsatellite analysis is a efficient invasive method forearly detection of bladder cancer and supervising its recurrence. Themeasurement of PTEN and nm23-H1 at the same time may have a guidingsignificance in BTCC.