| ObjectiveIn portal hypertensive rat's models, to investigate the changes of angiotensin- II (Ang- II )concentration, transforming growth factor (TGF- β ), extracellular signal-regulated kinase1/2 (ERK1/2) protein expression and distribution, procollagen I and III mRNA expression. By evaluating influence of Ang- II on TGF- β and ERK1/2 protein expression and distribution, to investigate the role of Ang- II in portal hypertensive vasculopathy. MethodsRats were divided in three group radomly : Portal hypertensive group, Portal hypertensive + Losartan group and Sham operate group. Detect the concentration of Ang- II with radio-immunity method. Analysis TGF- β and ERK1/2 protein expression and distribution with immunohistochemistry method. Analysis with image -analysis system,semi quantification of expression with RT-polymerase chain reaction method. Splanchno-vascular was studied with HE stain method and observed with microscope. All values are mean ?SE Comparisons between means of multiple groups were analyzed by one-way ANOVA and Scheff e's multiple comparison tests. Result: 1. SO(Sham Operate) group: The concentration of Ang-2 was : 14.79 +0.87pg/ml. Image-analysis Integra luminance worth was: TGF- :765.12 15.61, ERKi/2.' 562.47 + 23.29. Semi quantification procollagen I and mRNA was: procollagen I 77.89+2.47, procollagen III 72.07+2.41.2. PH(portal hypertension) group : The concentration of Ang- was :327.27 + 9.03pg/ml. Image-analysis Integra luminance worth was:TGF-P : 1718.51 ?7.93, ERKi/2: 862.7343.76. Semi quantification procollagen I and III mRNA was: procollagen I 172.97 ?2.74, procollagen III 129.73 ?3.71.Compare with SO group all above-mentioned data there were significant difference (P<0.05).3. Losartan group:The concentration of Ang-II was: 312.35 ?5.95pg/ml. Image-analysis Integra luminance worth was:TGF- 3 :1039.35 ?15.47, ERKi/2:705.32 + 46.38. Semi quantification procollagen I and III mRNA was: procollagen I 123.39 + 2.91, procollagenlll 88.26.37.Compare with SO and PH group all above-mentioned data there were significant difference (P<0.05).Conclusion:In portal hypertensive rats vasoactive substances such as Ang- II induce an increase in the procollagen I and III mRNA expression. Furthermore,we have shown that effects of Ang- II on portal hypertensive vasculopathy are very complex. Probably implicating the release of one hormone by the other and the systhesis of growth factor such as TGF- ERKj/2 and result in portal hypertensive vasculopathy happen.The striking inhibitory effect of Losartan in Ang- II induced increasing of TGF- ERKj/2. Procollagen I and III expression indications of Ang- II rceptor antagonist Losartan has therapeutic role in those situations.
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