Level Changes And Significance Of NO And ET-1 In Gastric Mucosa Of Portal Hypertensive Rats Before And After Pericardial Devascularization

Objective：To study the Level changes of NO and ET-1 in gastric mucosa of portal hypertensiverats before and after pericardial devascularization, and investigate the significance of NO andET-1 in porta lhypertensive gastrapath on the model of portal hypertension in rats.Methods：Male Wistar rats were randomly divided into three groups:10 rats in prehepatic portalhypertension group(PHPH), 10 rats in pericardial devascularization group(PD) and 10 rats insham operation group(SO).The prehepatic portal hypertension models were established bypartlyligation of Portal vein and succeeded two weeks later. The successful models wereundergone devaseularization after 14 days respectively. The Level changes of NO and ET-1 ingastric mucosa of portal hypertensive rats were measured by nitrate reductase and enzyme-linkedimmunosorbent methods before andafter pericardial devascularization for two weeks. Free portalpressur was measured Before taking a tissue every time.Results：1. The free portal pressure in prehepatic portal hypertension group was significantly higherthan that in the sham operation group and in pericardial devascularization group (P<0.01)，and inPD group was significantly higher than that in SO group(P<0.05).2. The density Level of NO and ET-1 in PHPH group was significantly higher than that inSO group and in PD group (P<0.01)，and in PD group was significantly lower than that in SOgroup(P<0.05).Conclusion：1. NO and ET-1 May be involved in the portal hypertension the happening of thestomach.2. Pericardial devascularization in a rat not only reduces the portal vein pressure, alsoreduce the inducing factors of NO and ET-1 in the portal hypertension stomach.3. Devascularization can maintain a balanced relationship between ET and NO in patients with portalhypertensive gastropathy...