| Objective To investigate the protective effect of combination of fructose diphosphate and polymyxin B on rat liver transplantatioan ischemia -reperfusion injury.Methods The models of orthotopic liver transplantation were establishedin rats. One hundred and twenty male rats were randomly divided into fivegroups: group A (sham-operation group) , group B (control group) , group C(fructose diphosphate group) , group D (polymyxin B group) ,and group E(fructose diphosphate and polymyxin B group) . Four animals of every groupwere killed at 1st, 6 th and 12th hours after transplantation. After collecting thefresh liver tissue sample and blood , the histopathologic alterations wereobserved under light microscope, serum ALT, AST, LDH and HA wereexamined.Results The values of serum ALT, AST, LDH and HA in group A were higer than normal level at a certain extent; all above-mentioned indexes in group B were significantly higher than in group A ; comparing with group B, all above-mentioned indexes in group C, D and E were significantly lower ,but significantly higher than in group A; there were no significantly differences between group C and D, but all above-mentioned indexes in group C and D were significantly higher than in group E. The histopathologic alterations under light microscope in group A were basically normal; in group B , along with the time going on, the histopathologic changes gradually aggravated, the changes included different degrees hepatic congestion, configuration destroy of hepatic sinusoid, degeneration and necrosis of liver cell; the changes mentioned underlight microscope in group C\ D and E were milder than in group C.Conclusions The combination of fructose diphosphate and polymyxin B had an obvious protective effect on rat liver transplantatioan ischemia -reperfusion injury. |
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