Experimental Study Of The Applying Of Bovine Brain Extract And Nourishing Piyin Remede On Rat Spinal Cord Injury

Background and Objective: The incidence of spinal cord injury is approximate 15~40 per million annually in different countries. The main reasons that lead to acute spinal cord injury are: traffic accidents, dropping from high places, and so on. The injury often results in the losses of motor, sensory functions below the injured level, that is to say, it brings about large burden on person, family and society. So the treatment of spinal cord injury has a meaningful sense. Spinal column fracture combining with spinal cord injury is a common disease and the process of pathology is complex. Nowadays, modern research concludes that there are two mechanisms that cause SCI: primary mechanism and secondary mechanism. While the treatment is mainly focus on the latter one. Neurotrophic factors can improve the growth functions of nerve cell and protect the injured neurons. Chinese traditional medicine deems that SCI is linked with "Tiduo" and "Weizheng", so the way to improve SCI is to activate the blood, improve gas and so on. Our experiment aims on the effects and possible mechanisms of bovine brain extract (bBE) and nourishing Piyin Remede (nPR), also tries to find a better solution.Methods: Ninety-six healthy SD rats were divided into 6 equal groups randomly: bBE supplied through subarachnoid cavity (A group), NS supplied through subarachnoid cavity (B group), bBE supplied through abdomen cavity (C group), NS supplied through abdomen cavity (D group), nPR oral taken group (E group), NS oral taken group (F group).Each group has 16 rats and the observing time is 12 weeks. Animal models of incomplete injury of spinal cord were made by Allen's equipment and a force of 10g×2.5cm on T8~ T9 segment. The force can make a mediate injury on spinal cord through a previous test. Furthermore, A and B group should place a microtube into the subarachnoid cavity. The whole process of model establishing should be aseptic. The BBB score and Rivlin plate, SEP, retrograde and label technique of Horseradish Peroxidase, gross observation, histological and morphological analyses were taken as the observed indices. Results: The value of observed indices of bBE supplied through subarachnoid cavity group, bBE supplied through abdomen cavity group, nPR oral taken group compared with their own control group improved evidently; the value of observed indices of bBE supplied through subarachnoid cavity group compared with bBE supplied through abdomen cavity group enhanced greatly.Conclusions: Bovine brain extract can stimulate the improvement of injured nerve fibers no matter it is used locally or generally, however, the former is better. Nourishing Piyin Remede can hold back the secondary spinal cord injury and accelerate the recovery of spinal nerve function.