| Objective: To observe neural protective effect of leave ginkgo biloba (EGb) after the spinal cord injury (SCI) in Sprague-Dawley (SD)rats. Methods: Modified Allen impaction methods were used in the present study to establish SCI models in SD rats. After anesthesia by 4% sodium pentobarbital (40mg/kg) through intraperitoneal injection, spinal segment of T8-10 was exposed by operation. Then the SCI was reduced at T9 by adjusting the height of the weight drop 5.0g to 5 cm above the exposed spinal cord to create spinal cord injury. Rats were randomLy divided into two groups. One group of rats were treated with normal saline solution, while the other group were administrated EGb 0.75 ml/ (kg·d) through intraperitoneal injection. Rats were sacrificed at 1d, 3d, 5d, 7d, 14d after the injury. The cell apoptosis of the injured spinal cord was examined by the Flow Cytometry. Expression of iNOS of the injured spinal cord was determined by immunohistochemistry. Result: Positive expression of apoptotic cells and iNOS appeared in both groups and reached their peak on 5rd day after the injury. Both the cells' apoptosis and the expression of iNOS of the EGb treated group were significant lower than that of the control group (p<0.05). Conclusion: This study demonstrated that EGb seemed to be possible to have neuroprotective effects on the experimental injured spinal cord and decreased the neuronal apoptosis and inhibited the expression of iNOS. EGb may become a promising neural protection drug in the treatment of SCI.
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