A Study On The Light Scattering Of Humen's Red Blood Cells And Monte Carlo Simulation

Hemorheology is a cross subject developed in 1960s. The blood cells can be studied by light scattering methods if considering them as particles of which diameters between several μm to hundreds μm.The paper studies light scattering of red blood in static status and deformed ellipsoid status caused by shear stress in Couette flow (get the values of three axis of red blood cell with light scattering). Monte Carlo simulation of photon's random movement in RBC suspension is also been investigated. The calculation of light scattering based on classic Mie theory is quite complex. So Fraunhofer diffraction theory, which is its approximate theory, is used in analysis of particles. The comparative refractive index of biological cells is near 1 and the error based on Fraunhofer theory is large because it doesn't take the light transmitted through the cells into consideration. This paper introduces another method of anomalous diffraction approximation to analyze the light scattering of static and deformed red blood cells according to the size and comparative refractive index. The theory takes the transmitting light into account and it is approved by simulation that the errors of the axises of RBC are smaller.The paper studied blood cells' light model and set up symmetrical and V/S model according to the light characteristics of RBC. further researches on light scattering of the RBC is based on these models. Monte Carlo simulation is a random trial method . The paper takes RBC suspension as random medium, RBC as scattered (absorbed) units. So tracing every photon's locus got statistic of light scattering of RBC. Then the values of three axises of RBC was obtained by inversing the anomalous diffraction . The simulation result shows that it is more suitable to analyze biological cells by anomalous diffraction as their compared refractive index close to unity.The last part of paper analyzed and compared the experiment data with simulation data, and proved that the correctness and applicability of the simulation method and anomalous diffraction theory.