P₁₆ Protein And VEGF Associated With The Biological Behavior Of Gastric Carcinoma

In order to study the expression and dependence of the P16 albumen and vascular endothelial growth factor (VEGF) in stomach cancer, use S-P immunity to organize the chemical method and quantitative analytical method of form, measure 78 stomach cancer and sample, stomach benign P16 albumen and expression of VEGF in the 15 tissues of pathological change, probe into it in taking place, developing function and dependence of the two of stomach cancer.The material and method: Collect 78 university first, two of Jilin, three hospitals of stomach cancer and sample excised in operation in 2001-2003 year of department of general surgery. Have not accepted chemotherapy or radiotherapy before patient's skill, has passed pathological diagnosis as the stomach cancer. Gland 32 of cancer among them, mucus gland 30 of cancer, low to split up 9 cancers, not 7 cancers of division; Fetch gastro scope is it examine 15 stomach benign pathological change (shallow form 7 of gastritis, 8 of gastritis of withering etc.) sample is it contrast group to make to live separately. PTNM is by stages (the international anticancer alliance IUCC announced the phased standard of the stomach cancer in 1997): ⅠIssues of +Ⅱ issues of 48, Ⅲ issues of +Ⅳ a issue of 30. 46 man, 32 women; 41 years old- 75 years old, average 56 years old. The diameter 1-6cm of tumor, the lymph gland has 34 persons who shift, there are no 44 persons who shift. All samples draw materials and treated according to the standard, prepare against the routine HE dyeing and immunity and organize chemistry to be dyed. Organize chemistry and dye the reagent in immunity: The mould of the chain resists the biotin - cross the immune group of oxidize and dye the ultra quick reagent box (Ultra Sensitive S-P kit), the monoclonal antibody of CD34, the monoclonal antibody of monoclonal antibody of VEGF and P16 buys from Fuzhou and steps the new biological technology development co., all antibodies have been already the using type; The antigen is repaired to buffer the liquid (the citric acid salt of 0.01M buffers hydrogen ion index 6. 0 of the liquid ); Job liquid . Organize chemistry to be dyed and adopted S-P law in immunity. Immunity organize chemistry dye course, substitute by PBS and resist and act as negative contrast, with known VEGF being positive and P16 positive stomach cancer organization cut into slices and make positive contrast. Judged finally: The pale brown particle appears with tumor cell and blood vessel endothelial cell afterbirth thick liquid, afterbirth membrane or afterbirth core separately, and its painted person who higher than background of intensity judges for being positive. Quantitative method of the form: 1, VEGF metering method: What VEGF express judge in order to must dye intensity and positive cell's percentage and assess. (a) Dye the intensity: It is 0 to be negative, it is 1 to be weak and positive, it is 2 to be positive, it is 3 to be strong and positive; (b) Positive cell's percentage: There is no positive cell that is 0, Neither for 1 in <25% nor for 2 in 26-50%, >50% for 3,a sum of +b is for 6, regard as negative by 0-2, 3-6 is regarded as the masculine gender. 2, P16 metering method: Each one cuts into slices and chooses 5 high power mirror visions; fetch its average after counting the positive cell and counting. Average every vision positive cell count percentage greater than 10% positive, less than or equal to 10% negative. Statistics is dealt with: Adopt four doses of forms χ 2 to examine.Result: The positive expressing rate of P16 is 48.72% in 1.78 stomach cancers (38/78), express in the afterbirth core of the tumor cell mainly, pale brown particle, some afterbirth thick liquid is expressed too. P16 expresses with the age, size of tumor, organize the credit type, splits up the degree has nothing to do (P> 0.05), and closely related with (P with tumor position, lymph gland transformation clinically by stages <0.01). 2.In 78 stomach cancers, the positive expressing rate of VEGF is 75.64% (59/78), its positive position is mainl...