Curative Effect And Mechanism Of Yan Shu Injector On Treating Malignancy Of The Older Adult

Objective:To study the curative effect and mechanism of Yan Shu injector on treating malignancy of the older adult, and to observe the change of the kill rate, cell cycle and apoptosis of turner cell treated by Yan Shu injector in vitro.Methods:60 cases of malignancy were divided into observational group(40 cases) and control group(20 cases).The observational group were treated by Yan Shu Injector cooperated with chemical therapy; the control group were treated only by routine chemical therapy.Clinical effect and side effects of both group were studied. In experiment, to observe the kill rate by MTT, PI mono-staining were used to detect the change of cell cycle and apoptosis rate, fluorescence staining to detect the expression of bcl-2 of HepG2 and SGC7901. Results: Effective rate of observational group is 50 percent, while that is 40 percent of control group(P>0.05).Side effects after chemical therapy of observational group is much less than control group(P<0.05 or 0.01).As to observational group, the quality of living, living time, the lenitive complexion and the immunologic indexes are all better than control group(P<0.05 or 0.01).To HepG2 and SGC7901,the living rate is 72% and 40%,the cell quality in S phase decreased more than the control, but the expression of bcl-2 has little change. Conclusion: Yan Shu Injector can alleviate the side effects of chemical therapy, lentitude ache, strengthen the curative effect of chemical therapy, improve the quality of living, prolong living time.lt is the same with the sufferers of malignancy; regulation of immunity is one of it's functional mechanism. We also find that, to HepG2 and SGC7901, Yan Shu injector can kill them, arrest them in 62 phase, block the conversion to M phase, accelerate cell apoptosis which has no correlation with the expression of bcl-2.