Experimental Study On The Tumor-inhibition And Anti-angiogenesis Effects Of Biejiajian Pills

Objective: To study the anti-tumor effect of Biejiajian pills to clarify the mechanisms on the anti-tumor effect of the decoction.Methods: Establish mice bearing neoplasm (H22 cell line) as the experimental tumor models. The mice were randomly divide into four groups: negative control group, positive control group, high-dose group of Bjj P, low-dose group of Bjj P. The mice of high-dose and low-dose groups of Bjj P were fed with Bjj P. In positive control group use cytoxan to inject into the mice's abdominal cavity. In negative group the mice were fed with distilled water. Treat the mice for fifteen days, during the 15 days the volume of the neoplasm block of all the mice bearing neoplasm were measured every three days. On the fifteenth day of treatmeat, the mice were killed all. Record the changing of the mice body weight before and after drug treatment. After stripping and weighing thymuses and spleens of all the mice bearing neoplasm, calculate the coefficient of thymus and the coeffcient of spleen. After striping and weighing the neoplasm block, calculate the inhibition rate of the anti-tumor. Then the tumor tissues were fixed with 10% formaldehyde. After the treatmeant of H&E staining, observe and count MDC of the neoplasm block of all the mice bearing neoplasm through light microscope and through the immunohistochemistry way measure the expression intensity of VEGF and PCNA.Results: 1 .The weights of the neoplasm of the medicine-feed groups were significant lighter than negative control group's (P<0.01). Compared with low-dose group of Bjj P, the weights of the neoplasm of high-dose group ofBjj P were significantly lightened ((P<0.05). Between of high-dose group of Bjj P and CTX group, the weights of the neoplasm had no significant difference(P>0.05).The tumor inhibition rate of CTX group was the highest. 2. Before dealt with the medicines, the weights of the different groups' mice had no significant difference ((P>0.05). After dealt with the medicines, compared with CTX group the weights of other groups' mice were significantly increased(P<0.01). Compared with negative control group, the weights of high-dose and low-dose groups of Bjj P were also significantly increased(P<0.01). 3.Compared with CTX group, the coefficient of thymus and spleen of other goups' mice bearing neoplasm were significantly increased(P<0.01). Compared with negative control group, the coefficient of thymus and spleen of high-dose group of Bjj P were significantly increased (P<0.05). 4.Compared with negative control group, MVD of high-dose and low-dose groups of Bjj P were significantly decreased(P<0.01), however, MVD of CTX group was not significantly decreased(P>0.05). Compared with low-dose group of Bjj P, MVD of high-dose group of Bjj P was significantly decreased(P<0.01). 5.Compared with negative control group, expression of VEGF of other groups were significantly decreased(P<0.01). Compared with CTX group, expression of VEGF of high-dose and low-dose groups of Bjj P were significantly decreased(separately P<0.01, P<0.05). Compared with low-dose group of Bjj P, expression of VEGF of high-dose group of Bjj P was significantly decreased(P<0.01). 6.Compared with negative control group, expression of PCNA of other groups were significantly decreased(P<0.01). Compared with CTX group, expression of PCNA of high-dose and low-dose groups of Bjj P were significantly decreased(P<0.01). Compared with low-dose group of Bjj P, expression of PCNA of high-dose group of Bjj P was significantly decreased(P<0.01). Conclusions: Bjj P can significantly inhibit the growth of the neoplasm of the mice bearing neoplasm. There is some dose-effect trend between high-dose and low-dose groups of Bjj P. Bjj P can also increase immune functoin of the mice, and decrease the tumor's dysfunction and inhibition tothe mice's immune organs. Bjj P can significantly decrease MVD of the neoplasm block of the mice bearing neoplasm and Bjj P can significantly inhibit the expression of VEGF and PCNA of the neoplasm block of the all mice bearing neoplasm. Ther...