Expression Of VEGF And PCNA And Microvessel Density In Osteosarcoma

Quantification of tumor angiogenesis has been recently noticed in tumor growth, metastasis and invasion, and became a "hot spot" in tumor research field. Angiogenesis has been proved essential for tumor growth, invasion and metastasis, such as breast carcinoma, prostatic carcinoma, liver cancer and so on. But there have been few studies of the role of the angiogenesis in human osteosarcoma. Its role is still controversial.The aim of this work is to study the role of the angiogenesis in osteosarcoma growth in and metastasis Immunohistochemical staining, using monoclonal antibodies was used to quantify the VEGF expression, the PCNA expression and the microvessel density in 52 surgical specimens resected from patients with osteosarcoma. Anti-VEGF monochonal antibody was used to determine PCNA expression and anti-factor VIII antibody was used to determine microvessel density.The result of research is listed:l.VEGF expression and MVD have significant correlation with metastasis (P<0.05).VEGF expression and MVD have no correlation with tumor cell-type and tumor size(P>0.05).2.PCNA expression has correlation with osteosarcoma metastasis(P<0.05)and There was no correlation with the tumor size and cell-type(P>0.05).3.MVD was significant higher in patients with the positive expression of VEGF than those with the negative expression of VEGF(P<0.05)4.PCNA expression was significantly stronger in patients with the positive expression of VEGF than those with the negative expression of VEGF(P<0.05).The result suggest that that angiogenesis is not only the basis of tumorgrowth but also the important factor in tumor metastasis. VEGF is associated with tumor angiogenesis and plays an important role in tumor growth and metastasis. MVD is essential factor in tumor growth and metastasis. The active proliferation of tumor cell increases the chance of metastasis. MVD and PCNA expression could be an important factor in the judgement of postoperation matastasis and prognosis and prognosis. Anti-angiogensis therapy can inhibit the growth, and metastasis of osteosacoma.