Study Of B Lymphocytes From Patients With Idiopathic Thrombocytopenic Purpura

Objective: To examine the quantity and apoptosis-related protein level of B lymphocytes in peripheral mononuclear cells in patients with idiopathic thrombocytopenic purpura (ITP) and to explore the role of B lymphocyte in the pathogenetic mechanism of ITP.Methods: We assayed quantities of all B lymphocytes and CD5+ B lymphocytes and expressions of CD95+ (Fas), and bcl-2 protein in B lymphocytes from 30 ITP patients and 20 normal controls by flow cytometry.Results: The percentages of B lymphocyte of lymphocytes is (11.43 2.71)% in ITP Patients, but that is (6.15 1.41)% in normal controls. Between of them there is a significantly difference (P<0.05). The percentages of CD5+ B lymphocyte is (19.76 1.58)% in ITP Patients, but that is (10.84 1.32)% in normal controls. Between of them there is a significantly difference (P<0.05). In ITP patients the expression of CD95+(Fas) protein is (2.01 0.85)%, and that is (1.58 0.64)% in normal controls. Between of them there is no striking different (P>0.05). In ITP patients the expression of bcl-2 in B lymphocytes is (81.74 14.25)%, but that is (53.33 9.57)% in normal controls. The expression of bcl-2 in B lymphocytes is significantly higher in ITP patients than in normal controls (P<0.001), Between of them there is a significantly difference.Conclusion: The abnormal quantities of B lymphocyte and its subsets and the inhibition of B lymphocyte apoptosis in peripheral monoculear cells is possibly relation to pathophysiology in ITP.