Isolation,Cultivation Of Neural Stem Cells Of Embryo Mice-derived And Differentiation In Co-culture With Mesencephalic Cells

Objective: To explore the natural differentiation of neural stem cells and the induced differentiation of neural stem cells by co-culture with mesencephalic cells.Method: 1. Cortices of embryonic mice were dissociated and single cell suspensions were achieved in sterile conditions. Then the cells were cultured in DMEM/F12 supplemented with bFGF and B27. Immunocytochemical techniques were used to identify stem cells and their progeny. 2. After labelled by DAPI, neural stem cells were co-cultured with mesenphalic cells. Immunocytochemical techniques were used to identify the TH-immunoreactive cells differentiated from the neural stem cells.Result: 1. In the serum-free medium containing bFGF and B27, the cells from embryonic mice proliferated in vitro, in which nestin antigen was expressed. In the medium containing serum but neither bFGF nor B27, the cells differentiated into neurons and glia. 2. Having co-cultured with mesenphalic tissue, a few of cells per neurosphere showed TH-immunoreactive.Conclusion: Neural stern cells from the cortices of embryonic mice can be cultured, passaged and differentiated into dopaminergic neuron by co-culture with mesenphalic tissue.