The Correlation Of The Co-expression Of MMP-2 And BFGF Between Tumor Angiogenesis In Plemorphic Adenoma And Adenoid Cystic Carcinoma Of The Salivary Glands

The angiogenic process can be summarized as follows:A cell activated by a lack of oxy- gen releases angiogenic molecules that attract inflammatory and endothelia! cells and pro- mote their proliferation;During their migration, inflammatory cells also secrete molecules that intensify the angiogenic stimuli;The endothelial cells that form the blood vessels respond to the angiogenic call by differentiating and by secreting matrix metalloproteases (MMP), which digest the blood-vessel walls to enable them to escape and migrate toward the site of the angiogenic stimuli;Several protein fragments produced by the digestion of the blood-vessel walls intensify the proliferative andmigratory activity of endothelial cells, which then form a capillary tube by alteringthe arrangement of their adherence-membrane proteins.;Finally, through the process of anastomosis, the capillaries emanating from the arterioles and the venules will join, thus resulting in a continuous blood flow. Angiogenesis performs a critical role in the development of cancer. Solid tumors smaller than 1 to 2 cubic millimeters are not vascularized. To spread, they need to be supplied by blood vessels that bringoxygen and nutrients and remove metabolic wastes. The clinical relevance of angiogenesis, as assessed by microvessel density (MVD) with an anti-CD34 monoclonal antibody. More than 20 endogenous positive regulators of angiogenesis have been described, including growth factors, matrix metal loproteinases, cytokines, and integrins. Growth factors, such as vascular endothelial growth factor, transforming growth factors, fibroblast growth factors (FGF), epidermal growth factor,angiogenin, can induce the division of cultured endothelial cells thus indicating a direct action on these cells. Matrix metalloproteinases (MMPs) are a family of enzymes ,which are secreted by both tumor and stromal cells, involved in degradation of extracellular matrix. This degradation is key for metastatic and angiogenic processes. Gelatinases MMP-2 and MMP-9 are expressed during carcinogenesis and angiogenesis. An imbalance between MMPs and naturally occurring MMP inhibitors may cause excess extracellular matrix destruction, allowing cancer cells to invade surrounding tissues and metastasize, and permitting angiogenesis to occur. Basic fbroblast growth factor (bFGF) is also a well-known angiogenic factor that stimulates cell proliferation, migration, and protease synthesis.Adenoid cystic carcinoma(ACC) of salivary gland origin is a types of salivary gland tumors, is highly invasive and frequently result in distant Metastasis. which is the main cause of treatment failure.The reasons for the invasiveness and aggressive metastatic dissemination of these tumors are uncertain. A possible mechanism may involve angiogenesis. This study is the first time that examined correlation of the coexpression of matrix metalloproteinase 2(MMP-2)and Basic fibroblast growth factor (bFGF) with tumor interstitial microvascular density(M VD) in ACC and PA.MethodsThe expression of FGF-2 and MMP -2 was examined in malignant salivary glandtumours(22cases), Salivary Pleomorphic Adenomas(14cases) and normal salivary glands(22cases) using the Streptavidin-biotin complex immunoperoxidase technique. microvessel density (MVD) assessed the clinical relevance of angiogenesis with an anti-CD34 monoclonal antibody.CD34 expression were detected in all case of ACC and PA. and nature salivary gland tissue by Streptavidin-biotin complex immunoperoxidase technique and was evaluated. SPSS was used to evaluate the relation of the co-expression of MMP-2 and bFGF between tumor angiogenesis in Plemorphic adenoma and Adenoid cystic carcinoma.Results(l).MVD in nature salivary gland tissue is 27.958.48:in Salivary Pleomorphic Adenomas is 45.43?6.01; in the Adenoid cystic carcinoma is 58.41 ?20. 52.CD34 expressed positivity was significantly different between the pleomorphic adenomas and Adenoid cystic carcinoma and nature salivary gland tissue(P<0.05). MVD in metastasis group of ACC is 6...