| Objective: Pityriasis rosea (PR) is a common, inflammatory skin disease with unknown etiology. Some scholars believed that PR was linked to viral infection (For example: human herpesvirus 6 and 7, cytomegalovirus and Coxsackie virus B et, al). F.Drago has found virus-like particles in both dermis and epidermis of PR lesions under the transmission electron microscope. But the contradictory findings have been reported by some other scholars. So it is controversial whether the etiology of PR is linked to a viral infection.The histologic features of PR include mild intracellular and intercellular edema in the epidermis, mild perivascular infiltration of lymphocytes and histocytes in the upper dermis. A perivascular extravasation of erythrocytes (even into the epidermis) may be present in some cases. Certain amount of red cells outside the blood vessels can be seen in some cases, however, only mild dilatation of blood vessels and endothelial edema are present and the structure of blood vessels is nearly intact in light microscope (LM). In this study, our purpose is to observe whether the abnormal particles related to viral infection are present in endotheliocytes of blood vessel and whether the ultrastructural structure of the endotheliocytes in PR lesions remain particular feature under the transmission electron microscope (TEM), further to research the etiology and mechanism of PR. The recent researches showed that cell immune reaction is related to PR, but by now, no detailed data can be provided to verify the significance between cell immunity and PR. Cell adhesion molecules are produced by cells, inducing the contact and combination between cells and between cell and matrices. VCAM-1(blood vessel cell adhesion molecule 1)and ICAM-1(intercell adhesion molecule 1) belong to immunoglobulin superfamily (IGSF).VCAM-1 mainly is expressed on the endotheliocytes of blood vessel. It combines VLA-4(very late activation antigen 4)on the leukocytes except neutrophilic granulocyte. Some cytokines can increase expression of VCAM-1. ICAM-1 is expressed extensively on the hemopoietic cell and other cells. It combines LFA-1 (lymphocyte function-associatd antigen-1) on the leukocytes. Both of VCAM-1,ICAM-1 on the endotheliocytes of blood vessel participate in the process that lymphocytes, monocytes, basophilic granulocytes and eosinophilic granulocytes penetrate the wall of blood vessel and reach the site of inflammation. ICAM-1 on the antigen presentation cell (APC) plays an important role in antigen presentation and activation of T cell as a co-stimulation molecule. In this study, our purpose is to explore the pathogenesis and mechanism of PR by detecting the expression of VCAM-1,ICAM-1 in the lesions of the patients with PR by immunohistochemical method .Methods: All samples were collected from the patients with PR, who were finally diagnosed clinically and pathologically and have not received any treatment after they were ill. All lesions and the normal skins were taken from trunk.8 patients with PR (male 4 cases, female 4 cases, age range from 15 to 34, course of disease from 3 days to 1 months) were obtained in order to observe the ultrastructural structure of PR under TEM, 2 normal skin samples as control. The samples were fixed in 4% Glutaral after obtained quickly, and then they were observed under TEM after being prepared in EM laboratory.22 patients with PR (male 12cases, female 10 cases, age range from 13 to 53, course of disease from 3 days to 2 months) were obtained in order to detect the expression level of VCAM-1,ICAM-1 in PR lesion (lesion group) and the adjacent normal skin (control groupâ… ) with immunohistochemical SP method , 10 nor...
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