| Objective:To confirm the inhibition of hepatic fibrosis and cirrhosis to Liver metastasis by animal experiments and clinical observation; to assess the role of Base fibroblast growth factor(bFGF), Platelet-derived growth factor-B, (PDGF-B), matrix metallo proteinase inhibitor-1 (TIMP-1) and P-selectin(P-S) in metastasis; to reveal the possible mechanisms about the inhibition of hepatic fibrosis to metastasis.Methods:1 Experiment of animals:1.1 Construction the mouse model of hepatic fibrosis:anosia inbred line BALB/c mice were randomly divided into two groups:normal liver group(group A, GA)and hepatic fibrosis group(group B, GB),32 mice in each group. BG was induced into hepatic fibrosis model by intragastric administration with carbon tetrachloride; 1.2 Cell culture:choosing Colonic adenocarcinoma cell line of BALB/c mice (CT26) to accomplish cell culture. 1.3 Construction of liver metastasis model:After the formation of hepatic fibrosis,we divided randomly GA mice into two groups:normal liver group(group A1, GA1) and normal liver inoculated with CT26 group (group A2,GA2)ï¼›GB mice was divided randomly into two groups:hepatic fibrosis group(group B1, GB1)and hepatic fibrosis inoculated with CT26 group(group B2, GB2).16 mice in each group. GA2 and GB2 were inoculated with CT26 and put to death after 10 days, observing metastasis stat. 1.4 Immunohistochemistry(IH):Using S-P technique,we detected the expression of bFGF,PDGF-B,TIMP-1 and P-S at groups A1,A2,B1,B2. 2.Clinical observation:collecting the case,dividing into groups and observing correlation index. Results:1 Experiment of animals:1.1 Results of liver metastasis model:After inoculating, lots of mice were body weight loss,hypokinesia and appetite reduction in GA2,but there were no obvious changes in GB2. GA2 and GB2 mice were put to death after 10 days, observing metastasis stat .The incidence of liver metastasis was 87.5%(14/16)in GA2,25%(4/16)in GB2, there was statistically significant difference between GA2 and GB2 (P<0.0001)(Table 1). 1.2 Results of Immunohistochemistry:1.2.1 bFGF:The positive rate of bFGF was 25% (4/16) in GA1,18.75% (3/16) in GA2,87.5% (14/16) in GB1,68.75% (11/16) in GB2 (Table 2). The positive rate of GB1 was higher than GA1,there was a statistically significant difference between the two group(P=0.001),The positive rate of GB2 was higher than GA2,there is a statistically significant difference between the two group (P=0.011).The expression of bFGF is seen in the liver of normal mice, but the expression level was very low. However, the expression of bFGF in the liver of hepatic fibrosis group was obviously high. There was no statistically significant difference between GA1 and GA2 (P>0.05) or GB1 and GB2 (P>0.05). There was no change on the expression of bFGF in the liver during metastasis. 1.2.2 PDGF-B (Table 3):The positive rate of GB1 was higher than GA1 (P<0.0001), the positive rate of GB2 mice is higher than GA2 (P<0.0001). There is no statistically significant difference between GA1 and GA2 or GB1 and GB2 (P>0.05).There was no change on the expression of PDGF-B in the liver during metastasis and the expression of PDGF-B in the liver of hepatic fibrosis group was obviously high. 1.2.3 TIMP-1:The positive rate of TIMP-1 was 31.25% (5/16) in GA1,25% (4/16) in GA2,87.5% (14/16) in GB1,81.25% (13/16) in GB2 (Table 4). The positive rate of GB1 mice was higher than GA1, there was a statistically significant difference between the two group(P=0.0032),The positive rate of GB2 mice was higher than GA2,there was a statistically significant difference between the two group (P=0.0038). The expression of TIMP-1 in the liver of hepatic fibrosis group was obviously higher than in the liver of normal. There is no statistically significant difference between GA1 and GA2 or GB1 and GB2 (P>0.05), however. 1.2.4 P-S:The positive rate of P-S was 12.5% (2/16) in GA1,87.5% (14/16) in GA2,6.25% (1/16) in GB1,25% (4/16) in GB2 (Table 5). The positive rate of GA2 mice is higher than G...
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