Effect And Mechanism Of Danshensu On Liver Injury Rats

Object: To investigate the protective role and pathogenesis of Danshensu(DSS) in liver injury.Methods: Acute liver injury was induced by intragastric Thionletamide(TAA). The rats were randomly divided into 4 groups which received the following treatmens: TAA+DSS group was made by intragastric DSS 7.2mg/kg for two days, from the beginning of the third day, TAA+DSS group and TAA group were made by intragastric TAA 250mg/kg for two days, at the same time, DSS group and TAA+DSS group were made by intragastric DSS 7.2 mg/kg for two days successively. After 24 hours the second intragastric TAA, blood was taken from abdominal aorta carefully. Liver function was evaluated by ALT,TB,LDH and endotoxin of plasma; Whole blood viscosity and platelet aggregation(MaxPag) will demonstrate the changes of hemorheology; meanwhile the liver homogenate lever of nitric oxide(NO) was evaluated by spectrophotometric determination and endothelin-1(ET-1) was detected radioimmunoassay. Weigert's staining showed microthrombi. The expression of endothelial nitric oxide synthase(eNOS) and inducible nitric oxide synthase (iNOS )were detected by immunohistochemistry method.Results: 1 The levels of ALT,LDH,TB and LPS in TAA+DSS group significantly decreased(vs TAA group ,P<0.05). The levels of NO and ET-1 in TAA+DSS group significantly decreased and NO/ET ameliorated. Whole viscosity and MaxPag(%) significantly decreased (vs TAA group ,P<0.05). 2 HE showed: infiltration of inflammatory cells in TAA+DSS group reduced and damage of hepatocytes significantly lighten(vs TAA group, P<0.05); hepatocytes inflated and sinusoidal narrowed, a large number inflammatory cells infiltrated and point or slice necrosis in TAA group. 3 Weigert's staining showed : Microthrombi in TAA+DSS group significantly reduced (vs TAA group ,P>0.05). 4 Immunohistochemical staining showed: eNOS expressial upregulation in DSS group compared with TAA+DSS group (p<0.05); nevertheless iNOS expressial upregulation in TAA group compared with TAA+DSS group (p<0.05).Conclusion: Intestinal endoxotemia(IETM) exit in the couse of occurring acute liver injury, which can induce the changes of hemorheology , microcirculatory dysfunction and the up-regulation of the hepatic iNOS expression. Danshensu could protect the liver from hepatic injury with TAA. The mechanism may be the decreasion of the value of intestinal endotoxemia, the amelioration of the liver function, the improvement of hepatic microcirculation and hemorheology and the up-regulation of the hepatic eNOS expression and down-regulation of the hepatic iNOS expression.