Effect Of Hypertonic Saline On Serum Levels Of TNF-α,IL-6,IL-10 In Rats With Severe Acute Pancreatitis

Objective To investigate the effect of hypertonic saline on the serum level of TNF-α,IL-6, IL-10 in an animal model for severe acute pancreatitis(SAP) . Methods SAP was induced in rats by intraperitoneal injection of 20 per cent L-Arginine . Animals were randomized into four groups ( each n=12 ) : (l)Controls ; (2)SAP intrarenous resuscitation with water ; (3)SAP plus intrarenous resuscitation with normal saline (0.9 per cent sodium chloride 2ml/kg ); (4) SAP plus intrarenous resuscitation with hypertonic saline (7.5 per cent sodium chloride 2ml/kg ) at 24h and 48h . Blood samples were collected at the 48h and 72h after SAP to measure serum levels of TNF-α,IL-6, IL — 10 and examine pancreatic histological changes . Results (1) Animals received hypertonic saline showed less pancreatic damage than those resuscitated withnormal saline . (2) Compared with those resuscitated with normal saline, serum levels of TNF-α, IL-6 of animals received hypertonic decreased significantly (p<0.05) and serum levels of IL - 10 increased more significiently at 72h after induce of SAP ( p<0.05) . Conclusions (1) Hypertonic saline resuscitation result in more effective method for reducing plasma levels of TNF-α, IL-6 and alleviating pancreatic damage. (2)Potential mechanisms for the beneficial effects of hypertonic saline may be include a effect of hyperosmolarity to reduce in endothelial cell swelling and so on, and depression in neutrophil activation.