The Effect Of Arsenic On Reproductive And Endocrine Hormones,COR And Cellular Immune Function In Female Rats

Objective The effects of arsenic on reproductive and endocrine hormones, COR and cellular immune function in female rats were studied. Methods Wistar female rats were randomly divided into 4 groups, One is control group(C), with distilled water, and three exposing groups, exposing dosages of arsenic were 0.28, 1.4, 7mg/kg/d; female rats were lavaged by body weights (lml/lOOg) for 10 weeks. The indexs were determined in the 10~th week. Results The results showed that (1) With the raise of exposing of arsenic, body weighs increased quickly in high dose group. The coefficients of heart liver spleen and kidney were all significantly increased. WBC and lymphocyte of the peripheral blood were increased too. T3 in high dose group were significant decreased (PO.01). The levels of T4 TSH and TNF in serum were not different among three exposing groups and control group. (2) The effect of arsenic on the level of estradio (E2), follicle stimulating hormone (FSH), luteinizing hormone (LH) and prolactin (PRL) in serum of female rats, and their levels of above mentioned hormones were determined by RIA. In spite of LH, there were not significant different of three hormones (P<0.05); (3) COR in serum of female rats in three exposing groups were lower than in control (P<0.01); (4) The counts of CD4+ cells and CD4+:CD8+ ratio in three exposing groups decreased than control (PO.01), having the tendency of gradual increase when increased the exposed dosage. The counts of CD3+ cells in three exposing groups decreased than control, only low exposing group was significant different, also having the tendency of gradual decrease with the exposing increase of arsenic. The counts of CD8+ cells were not significant difference among three exposing groups and control. Conclusion Under this condition, Arsenic have promote effects on the growth and development of female rats. With the raise of exposing dose of arsenic, the rats in highexposing groups could be developed toxic symptom, liver and spleen swell and cellular immune function were also inhibited. LH is a sensitive index for hormones of female rats, but it may directly damage the reproductive system.