Experimental Study On Anti-tumor Effects Of Apricot Polysaccharides From Xinjiang

Objective To investigate the anti-tumor effects and function of regulating immunity on S180-bearing mouse by Apricot Polysaccharides (AP) extract. Methods (1) Polysaccharides were extracted from Apricot through following steps: Removing fat and proteins, being drew in boiling water and deposited by ethanol. (2) MTT killing experiment, Bradford protein determination method, cell growth curve experiment and HE staining, fluorescence staining were used to study the effects of AP extract on tumor cell lines. (3) S180-bearing mouse were used to study the anti-tumor effects of both AP and co-administration of AP with Cyclophosphamide(Cy). Besides, T-cell mediated immunity was mainly observed. Results (1) There hardly have any proteins in crude AP extract after scanning through ultraviolet ray. Phenol-Sulphuric acid methods were used to determine the content of the extract, and the percent content of AP polysaccharides are 70.34% , the extract rate is 21.08%. ( 2) MTT, Bradford protein determination method showed that different doses of AP extract inhibited tumor cells growth, and dose of 10-1g/ml had significant growth inhibition on these cells, especially on Q3,HeLa cells. While AP had no inhibitory effect on protein synthesis of K562 cell. No inhibitory effect on normal liver cells was found. After HE,fluorescence staining, optics and fluorescence microscope showed that AP extract could induce apoptosis of Q3 and HeLa Cells. (3) Dose of 400mg/kg · d had inhibitory effects on tumor growth with inhibitory rate of 31.71%. The combined group had better effects with a rate of 46.98% than that of Cy group, which may contribute to AP enhancingimmune function and resisting Cy's damage to immunity. AP could promote cell-mediated immunity by accelerating T-cell proliferation and stimulating cytokine TNF- a in blood serum of tumor-bearing mouse at 400mg/kg · d. The combined group had better effects on promoting cell-mediated immunity. Conclusion Apricot Polysaccharide can inhibit the growth of carcinoma cell lines, probably by direct killing cells and inducing cell apoptosis. AP extract had inhibitory effects on S180-bearing mouse, which maybe the coordinated results of AP direct killing tumor cells and enhancing cell-mediated immunity. AP extracts also had the ability to lessen the toxicity of Cy and strengthen its function on tumor when co-administrated with Cy.