Study On Trace Element Levels And Chemical Species In Cancer Tissues And Side-cancer Tissues Of Patients With Lung Cancer

In this thesis, the levels of Cd, Pb, Cr, Mn, Ni, Cu, Zn in cancer and side-cancer tissues from 19 lung cancer patients and benign tissues from 9 lung patients were performed by using AAS. The results showed that the contents of Cu, Pb in cancer tissues were higher than in side-cancer tissues, and the contents in side-cancer tissues were higher than in benign tissues; Cd, Cr, Mn, Ni, Zn showed lower contents in cancer tissues than in side-cancer tissues. Pb, Ni may play an important role in the oncogenesis and development of lung cancer.Alkaline protease with 1% SDS was used to dissolve the lung powder. 70.48% of cancer powder and 79.31% of side-cancer powder dissolved. Approximate 3% of lead and nickel presented in the supernatant and the others in the residue. X-ray diffraction was performed to analysis the residues. The results confirmed the presence of NiO and Ni.Gel filtration chromatography of the hydrolysate on Sephadex G-75, Sephacryl S-300 HR columns showed a significant difference between cancer and side-cancer tissues. Lead chemical species in cancer tissues were few, whereas lead in side-cancer tissues had a variety of chemical species. Part of lead combined with biomolecules with molecular weight (3,000-70,000) in side-cancer tissues was transformed into biomolecules with higher molecular weight (>70,000Da). Part of nickel combined with molecules in side-cancer tissues was transformed into molecules with higher molecular weight.The results that two species NiO and Ni presented in the residues was very important in revealing the action mechanism of element Ni in the occurrence of lung cancer.