Establishment Of Pancreas Transplantation Model In Rats And Experimental Study On The Role Of Chemokines In Acute Pancreas Allograft Rejection

Part I Establishment of Pancreas Transplantation Model in RatsObjective To improve the method for pancreas transplantation and establish a physiologic rat model by the improved method.Methods Diabetes was induced by a single intravenous injection ofstreptozocin (STZ) at dose of 50mg/kg of body weight. The nonfasting blood glucose and urine glucose of the rats were assayed before the inducement of DM and measured on alternate day after the injection. Only rats with nonfasting blood glucose exceeding 16.8mmol/L and the strong positive reaction of the urine glucose were selected as recipients. SD rats served as donors and recipients. The vein was reconstructed by end-to-side anastomosis between the donor portal vein and the recipient superior mesenteric vein, and arterial reconstruction was carried out by end-to-side anastomosis of the donor to the recipient abdominal aorta. Enteric drainage is performed by a side-to-side anastomosis between the duodenum of donors and that of recipients.Results Of the 50 recipients, 39 survived more than 5 d with normal blood glucose. The successful rate of transplantation was 78%. Of the 11 that died, 7 died within 24h and 4 died within 48h after operation. The causes of death were thrombosis, pancreas graft necrosis, hypovolemic shock resultingfrom anastomotic leak, lung infection, narrowness of the venous anastomotic stoma. The donor operation consumed (51.88+5.12) min and the recipient operation consumed (63.10+7.18) min. The mean time used for the artery anastomosis was about lOmin, and that for the vein anastomosis was about 1 lmin. The mean cold ischemic time lasted about 45min.Conclusion This model of pancreas transplantation in rats is stable and reliable, which is in accordance with the trend of clinical pancreas transplantation and can be utilized for further scientific research. Part II Role of Chemokines in Acute Pancreas Allograft Rejection in RatsObjective To investigate the role of IFN-y inducible protein -10 (IP-10) and regulated upon activation, normal T cell expressed and secreted (RANTES) in the acute pancreatic allograft rejection in rats.Methods Diabetic SD rats were induced by streptozocin (STZ) firstly, and then pancreas transplantation was performed by a physiologic method of portal venous and enteric drainage. Two groups of rats underwent the transplantation, 24 cases in each group. Healthy SD rats served as donors and diabetic SD rats served as recipients in the isograft group (group A), healthy Wistar rats served as donors and diabetic SD rats served as recipients in the allograft group (group B). Six cases of diabetic SD and six cases of normal SD served as control groups. The serum IP-10, IFN-Y and RANTES were assessed by ELISA and the expression of IP-10 and RANTES in the allografts were determined dynamicly by immunohistochemistry at dayl, day4, day7, and day 10 post transplantation.