| IntroductionGentamycin which was consided as Aminoglycoside antibiotics can kill G~-bactaria. Gentamycin is a kind of ototoxic drug, which can bring about hearing loss. Many studies indicated that hearing loss derived from ototoxic drug was connected with oxygen free radical which make organ and cell of conchlea injuryed or be death. Gentamycin can make progress the form of oxygen free radical and add up the amount of oxygen free radical which can combine to creatine phosphate of hair cell. The combination can provide electronic donor needed by form oxygen free radical, bring about the most effective oxidation - reduction. The combination can obviously add up to contect nitric oxide with maleic dialdehyde in conchlea and make superoxide dismutase and glutathione peroxidase and cata-lase etal apparently consumpted, which result in organ and cell damaged. Some lipid peroxidation regarded as the transmitter of auditory nerve and hair cell make hair cell be death. Puerarin that is leguminous plant whose active principle is isoflavone was separated from pueraria root. There are some report, the chemical compound of isoflavone comprise reducing phenolic hydroxyl, thus can clear oxygen free radical, inhibit lipid peroxidation. Puerarin can only therapy sudden deafness in disease of ear. For the time being, we don't check out about report of puerarin in inhibiting the ototoxic side effects of gentamycin. It is necessary to study the transient ototoxic effects of gentamycin. In order to detemine whether puearin can inhibit the ototoxic side effects of gentamicin, we desigened a transient ototoxic animal model based on the injection of a middle single dose of genta-mycin and observed the electrocochleographic changes after administering the drug with or without simultaneous injection of puerarin . The results of studies indicated : puerarin as anti - oxidant, can protect the wistar rat cochlea from the ototoxic side effects of gentamycin. Puerarin may bring down the contect of cholesterol and triglyceride. Puerarin can inhibit the toxic reaction of sodium gluta-mate and N - Methy - D - aspartate.Materials25 healthy from 2 to 3 - mouth - old Wistar rats were randomly divided into three groups. The first group: normal control group have 5 rats ; The second group :GM group have 10 rats;GM + Pue group have 10 rats. The primary reagent and. instrument: Puerarin injection and Gentamycin injection and stereo-microscope Olympus BX51 and DPOAE instrument.Methods25 healthy from 2 to 3 - mouth - old Wistar rats were randomly divided into three groups . GM alone,were treated with GM 100mg/kg/d;by intraaabdominal injection, GM + Pue received GM 100mg/kg/d and Pue lOOmg/kg/d. Control group received injection daliy with the same volume of saline per kg body weight. Duration of treatment was 10 days, and the morphological and functional observations of cochlea were done. After the second DPOAE check, Wistar rats were killed, cochlaes were dissected with the aid of watch maker forceps and a stereomicroscope. Organs of Cortis were freed from surrounding tissues and were washed and fised for 3 hours in 4% paraformal. Specimens were examined under a stereomicroscope. The data were analyzed by Student's Test result.ResultChanges of DPOAE and hair cell were obvious in GM group, but only slight changes were found in GM + Pue group. There was remarkable difference in the GM group and Pue group period( p <0. 01). These changes were the same between the GM group and Pue group in the side of morphology. The DPOAE and morphology of Saline group were not change. In morphology, hair cell of GM...
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