Inhibition Effects Of Qingzhi Capsule On Platelet Activation And Aggregation In Rats With Attacked By LPS

Objects:to investgigate the influence of qingzhi capsule on platelet intraceller cAMP and cGMP levels , intraceller calcium of platelet ([Ca2 + ]i), plasma PGE2TXB2 and 6-K-PGF1α in two models of LPS attack in rats.Methods: Two kinds of model were established : after oral qingzhi capsule 8 days LPS attack was induced by intraperitoneal injecting (i.p.) LPS ; another model was established with high fat diet for 8 days, then induced by i.p. LPS at the last day. at 6h or 24h of post-supplementation rats were Anesthetized ,Blood was collected immediately from abdominal aorta. The level of Plasma TXB2 and 6-K-PGF1α was assayed by radioimmunoassay (RIA), cAMP and cGMP levels of platelet were assayed by RIA too. [Ca2 + ]i were examined with flow cytometer.the level of Plasma PGE2 was measured by enzyme-immunoassay(EIA).Results and Conclusion : Qingzhi Capsule increased the level of platelet [Ca2 + ]i on 24 hour after LPS attack , Qingzhi Capsule improved the levels of Plasma PGE2 and 6-K-PGF1α at 24h in the rats with only LPS attack . Qingzhi Capsule had strong effect on prevention of increasing of TXB2, Results also indicated depressing of TXB2/6-K-PGF1α ratio in the rats with high fat diet and LPS attack..