Expression Of Truncated Region Of S Genome Segment Of Hataann Virus In E.Coli And Preliminary Use Of This Recombinant Protein

Objective Cloning and expression of Hataann virus S gene truncated region(SA,Sh,Se) in Escherichia coli and using it in diagnosis of HFRS. Methods Extracted Hataann virus total RNA from the peripheral blood of acute HFRS patient by Trizol reagent, obtained target gene-SA by RT-PCR . Sequenced this fragment ,aligned it by NCBI Blast tools and found it belongs to Hataann virus. Applied GenBank accession number. Synthesizing and cloning truncated S gene of hataann and seoul using PCR. These genes were cloned into GST fusion protein expression vector pGEX4T-2, the recombinant protein was examined by western blotting with polycloned antibodies. Results The molecular weights of SA ,Sh,Se was 49 000D ,46 000D,37000D, and mainly expressed as inclusion body in Escherichia coli. Western blotting analyses demonstrated that this recombinant protein presented the specific antigenicity Conclusion These prokaryotic expression portion of the NPs of this specific hataannviruses can be used to generate readily and efficiently in diagnostic assays for HFRS.