Study On Correlativity Between PTEN Gene Mutation, Protein Expression And Endometrial Cancer

Objective:To study the relationship between PTEN gene mutation , protein expression and the generation,development,clinical pathologic characteristic of endometrial carcinoma.Methods:Polymerase chain reaction—single strand conformation polymorphism (PCR—SSCP) method and DNA sequencing technique were used to detect mutation of PTEN gene in 52 endometrial carcinoma tissues and 10 corresponding normal tissues. Immunohistochemistry was used to examine the protein expression of PTEN gene, and analysis clinical pathologic characteristic of endometrial carcinoma.Result:1. The mutation rate of the exon 5 and exon 8 of PTEN gene was 34.6%(18/52) .No mutation was found in 10 normal tissues. Statistical analysis showed that histological grade, histological type and depth of myometrial invasion had Significant relationship with PTEN gene mutation(P<0.05).2 .All normal tissues were found to express PTEN protein. PTEN protein expression deletion rate in 52 endometrial carcinoma tissues was 67.3%(35/52), significantly higher than that in the corresponding normal tissues(P<0.05). PTEN protein expression deletion was significantly associated with histological grade and type and muscular invasion(P<0.05), but was irrelevant to diabetes mellitus, obesity, hypertension (P>0.05).3.Among 52 endometrial carcinoma tissues, 17 cases displayed positive protein expression of PTEN,35 cases displayed negative protein expression. Among 35 cases of negative protein expression mutation was noted in 18 cases.Conclusion:1.PTEN gene mutation or expression loss of PTEN is an event in the endometrial carcinoma.2. PTEN gene mutations are usually found' in high histological grade, low muscular invasion endometrial carcinoma and endometrial endometroid carcinoma. PTEN gene mutations play an important role in the diagnosis; Expression loss of PTEN is usually found in low histological grade, deep muscular invasion endometrial carcinoma and endometroid carcinoma. Expressions loss of PTEN an important role in the endometroid carcinoma.3. PTEN gene mutation plays an important role in expression loss of PTEN.