Study On Immune Toxicity And Oxidative Damages Induced In Mice By Formaldehyde

Objective: Formaldehyde has been assessed as one of the main airpollution indoor with the rise of indoor decoration. It has been attached greatimportance to the healthy harm induced by FA. Immune injury might be one ofmain reasons which induced injury of tissue and organ by FA. Some studyrevealed that lipid peroxide was a kind of approach of resulting in immuneinjury induced by combination. The aim of this experiment was to studyimmune toxicity and lipid peroxide injury of mice induced by FA in a statictotal enclosure chamber and to provide overall realization of immune toxicityinduced by FA and to provide theoretic reference for realizing the poison of FA. Methods:30 KM mice were randomly divided into 5 groups as following:negative control group,1mg/m³,3mg/m³,5mg/m³ FA inhalation groups andpositive control group. The mice of FA inhalation group were exposed to FA byinspiration in a static total enclosure chamber 2 hours everyday for 14 days.The mice of negative control group breathed fresh air in the same condition.The mice of positive group were injected CP into paunch with 40 mg per kgbody weight for 7 days from the eighth day begin. According to documents, atlast, following tests were taken: The relative weight of spleen and thymus wascalculated; The contents of CD₃,CD₄,CD₈ of spleen and thymus were measuredwith immunocytochemistry; The swallow function of macrophage in mice wasmeasured with the CRBC swallow experiment; The delayed typehypersensitivity of mice, the quantity of antibody forming cell andlymphocyte function transformation were measured Separately by the typicalmethod. The activity of superoxide dismutase in spleen and thymus in micewas measured by hydroxylamine assay; The contents of maleic dialdehyde inspleen and thymus in mice were measured by improved colorimetric method.Results:1.The relative weight of spleen and thymus of mice weredecreased when mice inhaled FA vapor. There was a significant declinetendency of the relative weight of spleen and thymus of mice in FA inhalationgroup compared with negative control group(p<0.05 or p<0.01); 2. The resultshowed a significant decline tendency of content of CD3,CD4,CD8 of spleenand thymus of mice with dose increased. There were obvious significance in FAinhalation group compared with the negative control group (p<0.05 or p<0.01).There were dose-response relation between the content of CD3,CD4,CD8 ofspleen and thymus of mice with the quantity of formaldehyde inhabited. Thedegree of decline of CD8 was bigger than CD4 in all of mice; 3. The swallowfunction of macrophage had obvious significance in FA inhalation groupscompared with the negative control group(p<0.05) .4.Both lymphocyte functiontransformation of spleen and the quantity of antibody forming cell showed asignificant decline tendency with dose increased and had obvious significance inFA inhalation group compared with negative control group(p<0.05); 5.Thesignificantly lower activities of SOD in spleen and thymus in FA inhalationcompared with the negative control group(p<0.05 or p<0.01)except the 1mg/m3dose group in spleen. The content of MDA was increased significantly in FAinhalation group compared with the negative control group(p<0.05 or p<0.01).The activities of SOD in spleen and thymus in FA inhalation groups had anegative correlation with the dose of FA inhabited. Conclusion: The injury of immune pathology,humoral immunity, cellimmunity and nonspecific immunity of mice were resulted from FA vaporinhabited, and there was obvious dose-response correlation between thedegree of immune injury and the dose of FA ; FA may induced lipid peroxide inspleen and thymus of mice, and there was obvious dose-response correlationbetween the degree of lipid peroxide and the degree of immune injury ; LPOmight be one of mechanism of immune injury induced by FA.