| Non-Hodgkin lymphoma (NHL) is one of the hematological malignant tumors which endamage human health seriously. As we know the excessive proliferation and decreased apoptosis are the foundations of tumor development. Finding out some biological characteristics of tumor cells may be benefit to diagnosis, judgement of the aggressive degree, treatment and assessment prognosis of this disease and afford the new treat target.The core of cell cycle regulation and control mechanism are relative to a group of protein kinase. P27, a cyclin-dependent kinase inhibitor (CDKI) reported firstly in 1994 year. It was found that p27 protein was a negative cell cycle regulator and that the expression of p27 protein died down gradually in the tissues of precancerous lesions and early or advanced tumors. It has been shown that p27 is related with proliferation, differentiation, metastasis, and prognosis in some tumors. Thedownregulation of p27 protein was common in epithelial carcinomas, but that was not as same as in lymphomas. Survivin, as a cell cycle dependent gene can inhibit the apoptosis of tumor cells. There were an overexpression of survivin gene in embryonic tissues and many human tumors, but it'was undetectable in normal differentiated tissues.The aberrant expressions of survivin protein and p27 protein were related with the poor prognosis in some tumors such as breast, prostate, respiratory, digestive, cerebral cancer, etc. The expression levels of survivin protein and p27 protein in the tissues of non-Hodgkin lymphoma and their relationship with clinical pathological factors including location aggressive degree clinical stage, immunophenotype, and serum lactate dehydrogenase were assessed in this article.Materials and MethodsThe expression levels of survivin protein and p27 protein were measured in 41 cases of NHL from 2001 year to 2004 year in our hospital. There were female 19 cases and male 22 cases. Their age was 7 years old to 74 years old. None of the patients had received chemotherapy and (or) radiotherapy before biopsy and surgery. There were 18 cases happened in extronodal sites, 23 cases in intronodal sites in 41cases. The tissue sections were measured 4 u m from paraffin-embadded tissues and the morphologic examination was done on the hematoxylin-eosin staining samples by using microscope. The diagnosis was made according the criteria of the World Health Organization classification (2000 year) of lymphomas. Twenty samples of reactive lymphnoditis were used as controlgroup.The correlations between the expression levels of the survivin protein and p27 protein with clinical pathologic factors including age, location, the aggressive degree, clinical stage and immunophenotype were analyzed.The immunophenotype results were based on B-lymphocyte and T-lymphocyte markers includingCD3, CD20, CD79a, CD45R0, etc by immunohistochemical method. The positive expression of survivin protein in the cells assessed showed yellow or brown in the cytoplasm and the nucleus of cells, and the positive expression of p27 protein revealed brown in the nucleus of cells. The breast cancer cells were used as positive control group. PBS was replaced the primary antibody in negative control group. The positive expression percentage of survivin protein in the cells of NHL was calculated in five areas at 400-fpld magnification. The percentage <5% was defined as negative and that of 5% but <50% was positive(+) ; that of 50% was (++). As above method , the positive expression percentage of p27 protein in the NHL cells 10% was defined as negative, that of 10% was positive.Results1. The. expression level of survivin protein in the tissues of NHL.There were the positive expression of survivin protein in 39 of 41 cases (95. 1%), while none of 20 patients with reactive lymphoditis was positive. The positive expression percentage of survivin protein was 71.96% ± 30.90% in the cells of NHL. Among the positive expression of patients, the expression level' of survivin protein was higher in theaggressive group than that in the indolent group (P<0.05). The... |
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