The Study On Drain Dopamine And Serotonin Concents Of Uremia Encephalopathy Rat And On The Mechanism Of Uremia Encephalopathy

Objective: To detect the brain dopamine (DA) and serotonin (5-HT) contents of acute renal failure rats with nervous system complication and study the mechanism of uremia encephalopathy.Method: 36 female Wister rats were divided into 2 groups by random, 12 in control group,24 in model group.In the model group, Cisplatin 10mg / kg i.p. qd×2 to induce acute renal failure. According to their clinical manifestation, the model group was devided into uremia group and uremia encephalopathy group . Detecting the brain dopamine and serotonin contents by high performance liquid chromatography (HPLC); and detecting the serum creatinine (SCr).Result: The level of SCr in uremia group and uremia encephalopathy group was higher than that in control group, had significant difference (p<0.05). The level of SCr in uremia encephalopathy group was higher than that in uremia group, had significant difference (p<0.05) . The brain DA and 5-HT contents of uremia group were lower than those of control group ,didn't have significant difference (p>0.05) . The brain DA and 5-HT contents of uremia encephalopathy group were lower than those of control group, had significant difference (p<0.05) .The brain DA and 5-HT contents of uremia encephalopathy group were lower than those of uremia group, have significant difference (p<0.05) . The concents of two neurotransmitters didn't have linear correlation with SCr(r=-0. 347; -0.277, p>0.05).Conclusion: Cisplatin 10mg / kg i.p. qd × 2 may build acute renal failure succesefully. The brain DA and 5-HT contents had no change when acute renal failure without nervous system complication ,but grew lower when with nervous system complication., so DA and 5-HT may play a role in uremia encephalopathy . More higher...