| As the incidence rate and mortality rate of ovarian carcinoma werehigher and higher in the world, it now ranks worst among the malignanciesthat seriously threaten the women's health and lives in the most cities andregions of China. Because the most patients were diagnosed at late phase, itis poor effect and bad prognosis. Finding a new prognosis marker andmolecular therapeutic target for ovarian carcinoma are needed. Aurora kinase is a novel kinase that have been identified ascentrosome amplification, chromosome instability and cell transformationin the present research. Aurora-A is frequently amplified in many humancarcinomas such as colorectal, breast ,bladder and tongue carcinoma.Aurora-A gene has been identified as a novel oncogene due to itsoverexpression detected in many human tumors as well as overexpressionhas been shown to cause tumorigenic transformation of human and rodentcells in vitro and in vivo. To find the expression of Aurora-A in ovarian carcinoma, two ovariancarcinoma cell lines: HO-8910 (ovarian epithelial serouscystadenocarcinoma), HO-8910PM ( highly metastatic ovarian epithelialserous cystadenocarcinoma) in mRNA, protein level were examined bysemi-quantitative RT-PCR and Western-blot. The proportion of cells with4N and >4N DNA content in two ovarian carcinoma cell lines wereinvestigated by flow cytometry, the association between Aurora-Aexpression and DNA content was analyzed, and this study provides atheoretical basis for finding a new tumor marker and molecular therapeutictarget for ovarian carcinoma. The result of semi-quantitative RT-PCR was analysed with TotalLabsoftware ,and the ratio of Aurora-A/β-actin was 0.95(HO-8910),0.98(HO-8910PM),0.26(normal ovarian tissue),which were indicatedAurora-A mRNA was overexpressed in all two ovarian carcinoma linescompared with normal ovarian tissues, and the level of Aurora-A mRNAexpression was difference: HO-8910PM > HO-8910. The result of westernblot was also analysis with TotalLab software,and the ratio ofAurora-A/β-actin was 1.24 (HO-8910) and 1.58(HO-8910PM).The tworesults even indicate that two ovarian carcinoma lines were overexpressedin mRNA and protein level, furthermore those were concordant in theexpression level. Many researches have showed that the biology behavior of malignanttumor are associated with degree of aneuploidy abnormality. Those areworse in increased DNA content (mostly caused by polyploid) of malignantneoplasm and they are hyper invasive. Therefore it is aneuploidyabnormality that can evaluate some malignant neoplasm progression andbecome prognosis marker. The proportion of cells with 4N and >4N DNA content inHO-8910,HO-8910PM were 21.63%and 30.15%respectively, and thedifference of the proportion had statistical significance (p<0.01) .Theproportion with >4N content were 2.20 % ,7.16% respectively andHO-8910PM had significant difference with HO-8910 (p<0.01); The rankof the proportion of cells were HO-8910PM >HO-8910 in both 4N and>4N DNA content level. The proportion of HO-8910PM with 4N DNA content washighest ,which was related with the reason that it is a high metastasis cellline. The rank of the proportion of cells with >4N DNA content wereHO-8910PM >HO-8910 that was concordant with the rank of Aurora-Aexpression. So overexpression of Aurora -A has corralation with theproportion of ovarian carcinoma cells with DNA content (>4N). BecauseDNA content is a prognosis marker in ovarian carcinoma, Aurora -A andexpressed kinase may possibly be a new molecular marker to assess theprognosis of ovarian carcinoma. Aurora-A was overexpressed in two ovarian carcinoma cell lines, thelevel of Aurora-A mRNA and protein expression were different. The 4Nand >4N DNA contents were different between two ovarian carcinoma celllines. Overexpression of Aurora -A has corralation with the proportion ofovarian carcinoma cells with >4N DNA content. which was related with thereason that HO-8910PM is a highly metastatic cell line. The study suspectsthat ovarian carcinoma due to chromosome instability caused byoverexpression of Aurora-A. |
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