| Objective According to the expression levels of Aurora-A and Aurora-B kinases in gastric cancer tissues, atypical hyperplasia tissues and normal gastric tissues, to explore the synergistic effect of these two kinases in genesis and development of gastric cancer and to provide a theoretical basis for screening tumor marker and molecular therapeutic target of gastric cancer.Methods Protein levels of Aurora-A and Aurora-B in gastric cancer tissues, atypical hyperplasia tissues and normal gastric tissues were detected by immunohistochemistry and Western blot. Variability and dependability of Aurora-A and Aurora-B in gastric cancer with different degree of differentiation were analyzed.Results1.Aurora-A expression was observed in 0 case(0/40,0%) of normal gastric tissues, and in 15 case(15/25,60%) of atypical hyperplasia tissues, and in 30 case(30/40,75%) of gastric cancer(GC). Its expression in GC was much higher than that in normal gastric tissues (P<0.01) or atypical hyperplasia tissue (P<0.05). Its expression in atypical hyperplasia tissue was much higher than that in normal gastric tissues (P<0.01).2.The expressions of Aurora-A was associated with the degree of differentiation, which expression ratio was37.50%(3/8),70.00%(7/10),90.09%(20/22) in well-, moderately-and poorly-differentiated adenocarcinoma respectively. The expressions of Aurora-A increased when degree of differention degraded(P<0.05).3.One case(1/40,2.5%) of normal gastric tissues showed expressions of Aurora-B, however,13 case(13/25,52%) of atypical hyperplasia tissues and 28 case(28/40,70%) of gastric cancer showed expressions of Aurora-B (P<0.05).4.The expressions of Aurora-B was associated with the degree of differentiation. which expression ratio was 37.5%(3/8),60.00%(6/10),86.36%(19/22) in well-, moderately-and poorly-differentiated adenocarcinoma, respectively. The expressions of Aurora-B increased when degree of differention degraded(P<0.05).5.The expression of Aurora-A were positively with Aurora-B. But both of the expressions of Aurora-A and Aurora-B were not associated with age, sex and lymph node metastasis (P>0.05).6.Western blot was adopted to assess the status of Aurora-A and Aurora-B protein expressed in tumor in vivo from 22 patients. The over-expressin of Aurora-A and Aurora-B protein was detected in 12 cases (54.55%) and 10 cases (45.45%) compared with their neighboring tissue, respectively (P<0.05).Conclusions1.The Aurora-A and the Aurora-B was highly expressed in gastric cancer tissue than in neighboring tissue, and its increasing expressionwasconcerned with malignant degree, which suggests that the over expressed Aurora-A and Aurora-B be one of oncogenetic factors of gastric cancer.2.Positive expression of Aurora-A and Aurora-B suggests they commonly participate in genesis and development. Anormal expression of Aurora-A and Aurora-B play certain role in gastric cancerogenesis, and may become new target and tumor marker in molecular therapy on gastric cancer. |
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