Investigate The Inflammatory Marker And Coagulation Factors In Deep Vein Thrombosis

Objective: To investigate the correlation between C-reactive protein(CRP), CRP 1059 G/C gene polymorphism and deep vein thrombosis. The plasma level of factor VIII coagulation activity (FVIII:C), factor IX coagulation activity (FIX:C) and fibrinogen were examined, and the correlation between the level of plasma C-reactive protein as a marker of inflammation and fibrinogen, FVIII:C and FIX:C were assessed. To analyze the interaction between inflammation and coagulation in the development of deep vein thrombosis. Methods: The patients with deep vein thrombosis undergoing selective venous ultrasonography were enrolled in this study. The plasma levels of C-reactive protein was detected by immunoturbidimetry analyzer, FVIII:C, FIX:C were determined by a one-stage assay and fibrinogen was measured by full-automatic biochemical apparatus respectively in 59 cases of deep vein thrombosis and 26 controls. Polymerase chain reaction-restriction fragment length polymorphism was used for the screen 1059 G/C polymorphism in exon 2 of C-reactive protein gene in 61 cases and 60 healthy control subjects, the frequency of mutation was counted. Results: The mean levels of plasma C-reactive protein, fibrinogen, FVIII:C and FIX:C were significantly higher in deep vein thrombosis group than that in controls [C-reactive protein, (2.67±0.91) vs (0.14±0.08)(mg/dl); fibrinogen, (4.73±1.36) vs (2.79±0.66)(g/l); FVIII:C (126.71±28.10) vs (81.35±20.77)(%); FIX:C (81.01±23.60) vs (70.71±11.3) (%)], it showed significant difference (P<0.01), and the level of plasma C-reactive protein was strongly correlated with fibrinogen, FVIII:C and FIX:C (r s = 0.432, 0.571 and 0.544, P<0.01). CRP 1059G/C polymorphism was no statistical correlation between deep vein thrombosis group and control group (P>0.05). Conclusion: Deep vein thrombosis and inflammation were closely related, increased level of plasma C-reactive protein may be a predictor of deep vein thrombosis. Increased plasma levels of fibrinogen, FVIII:C and FIX:C all were important risk factor to deep vein thrombosis. In deep vein thrombosis, inflammatory reaction may probably promote thrombosis by elevating coagulation factors. 1059G/C polymorphism of the C-reactive protein gene was no association with deep vein thrombosis.