Investigation Of Effect Of Psoriatic PBMCs Culture Supernatant On HPP-CFCs In Vitro

Objective To observe influence of culture system on colony formation, study effect of psoriatic peripheral blood mononuclear cells (PBMCs) on activity of marrow high proliferative patential colony forming cell(HPP-CFCs) and reveal the relation of immunocytes and HPP-CFCs from psoriatic patients. Methods Marrow mononuclear cells were separated by density gradient centrifugalization and cultivated in methylcellulose semi-solid culture medium with cytokines and PHA-stimulated condition to establish idealer culture system for HPP-CFCs . The HPP-CFCs were cultured in the culture system stimulated respectively with PBMCs culture supernatant derived from psoriatic patients and normal controls, and the colony number of HPP-CFCs was counted on the 14th day after culture. Results HPP-CFC colonies formed in medium both with PHA-MCM and with cytokines, however the colonies were more in cytokines system than in PHA-MCM one(P<0. 05) . Compared respectively with the group of spontaneous proliferation and the group stimulated by PBMCs culture supernatant from normal controls, the HPP-CFC colonies decreased significantly (P<0.05); However, there was no statistical significance between the group stimulated by PBMCs culture supernatant from normal controls and the spontaneous proliferation group(P>0.05). Conclusion The cytokines culture system is superior to the PHA-MCM one; Colony formation of HPP-CFCs can be suppressed by psoriatic PBMCs culture supernatant, which suggest that psoriatic PBMCs have specific biological activity; The marrow microenvironment can be affected by peripheral blood environment , so the activity of psoriatic patients HSCs is probably abnormal.