| Objective: To investigate the effects of indole-3-carbinol (I3C) on theexpressions of Akt-1, NF-κB, Bcl-X/L, Bax and Bcl-2 proteins in carotidartery of rats after balloon lesions, and to study the molecular mechanismsof I3C inducing the apoptosis of vascular smooth muscle cells (VSMCs)after blood vessel injury, the study will provide a theoretical basis forpreventing coronary artery restenosis after percutaneous coronaryintervention (PCI) in using I3C. Methods: 35 SD rat models (weight: 300- 400g) with carotid lesionswere divided randomly into 2 groups: control group (balloon lesions buttreated with nothing, n=5) and experimental group (treated with I3C, n=30).The experimental group was subdivided into 6 groups by different doses(12.5mg/d, 25mg/d and 50mg/d respectively) and different treatment time(4 days and 7days respectively). 14 days later after operation, the targetsegments were acquired. Then the protein expression changes of Akt-1,NF-κB, Bcl-X/L, Bax and Bcl-2 were detected in the carotid wall cells withSABC method, and the mean optical density values of Akt-1, NF-κB andBcl-X/L and the mean scores of Bax, Bcl-2 were measured by computerimage analysis. Results: 1. Protein Akt-1 was mainly expressed in the middle layer of ratscarotid artery. The expression values of Alt-1 were increased in controlgroup, however, in experimental group, decreased in different degree,especially in 50mg 4-day-group, 25mg 7-day-group and 50mg 7-day-group(0.0725 ±0.00581 vs. 0.0590± 0.00235, P<0.01; 0.0725 ±0.00581 vs.0.0646 ±0.00257, P<0.05; 0.0725 ±0.00581 vs. 0.0517 ±0.00207, P<0.01).In the same doses of I3C (50mg/d), there were differences in 4-day-groupand 7-day-group (0.0590 ±0.00235 vs. 0.0517 ±0.00207, P<0.01). 2. Protein NF-κB were both expressed in the control group and theexperimental group, its positive particles can be found in cytoplasm ornucleus of rat carotid artery wall cells. In each sub-group of theexperimental group, the expressions of NF-κB were lower than thecorresponding control group. There were significant difference between thecontrol group and the experimental group (P<0.05 或 P<0.01) excepting the12.5mg 4-day-group (0.0947 ±0.00252 vs. 0.1003 ±0.00700,P=0.516).Comparison with the sub-groups of the experimental group in the samedoses and the different treatment time, there was only statisticalsignificance between the 25mg 4-day-group and 25mg 7-day-group (0.0872±0.00146 vs. 0.0816 ±0.00211, P<0.01). 3. Protein Bcl-X/L was mainly detected in the smooth muscle cellcytoplasm of rat carotid artery, and it located chiefly in the middle layersmooth muscle cell of the carotid artery. In the control group, the meanoptical density value of Bcl-X/L was 0.0809 ±0.00170, and the mean valuein the experimental group was decreased. There was significant difference(P<0.01) between the control group and the 50mg 4-day-group (0.0593±0.00111), 25mg 7-day-group (0.0766 ±0.00059) and 50mg 7-day-group(0.0516 ±0.00235). In the experimental sub-groups treated with the samedoses but not the same treatment time, there were not statistic differenceapart from the comparison of 50mg 4-day-group and 7-day-group (P﹤0.01). 4. Protein Bax were both expression in the control group and theexperimental group after balloon lesions in carotid artery of rats, and itlocated chiefly in the smooth muscle cells in the middle layer of the carotidartery. In each experimental sub-groups, the expression of Bax wasincreased more than the control group, especially in the 50mg 4-day-group,25mg 7-day-group and 50mg 7-day-group (5.8000 ±0.46368 vs. 2.4400±0.35777, P<0.01; 3.0600 ±0.18166 vs. 2.4400 ±0.35777, P<0.05; 5.9800±0.41473 vs. 2.4400 ±0.35777, P<0.01). In the same I3C doses, there wasnot significant difference in the expression of Bax between 4-day-groupand 7-day-group. 5. Protein Bcl-2 was decreased in the experimental group comparedwith the control group. There was significant difference between them withthe exception of 12.5mg 4-day-group (4.1200 ±0.47645) and 12.5mg7-day-group (3.9200 ±0.42071). In the...
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