| Allergic rhinitis(AR)is one of the common diseases in otolaryngology head and neck surgery.It is an IgE mediated allergic disease of nasal mucosa after body contact with allergen.The main symptoms of AR are nasal obstruction,nasal itching,paroxysmal sneezing and runny.Allergy is a growing problem throughout the western world.It is estimated that 40 percent of the EU’s population,mainly young people and children,are affected and their quality of life is often seriously affected.Allergic diseases include diseases with multiple factors,including genetic and environmental effects,and the composition of the microbiome.Many exogenous factors,such as increased exposure to indoor allergens,increased environmental pollution,dietary changes or breastfeeding,may contribute to increased allergic rhinitis.However,there is still a lack of clear relationships and evidence to demonstrate clear risk factors.There may be a link between Western lifestyle habits and the development of allergies,but this link is still in heated debate.Among the numerous pathogenic factors,T cells play a crucial role.Th1/Th2 paradigms have been dominant concepts over the past decades.Based on observations,patients with allergies tend to have an increased Th2 response to allergens.Allergic rhinitis is the Thl/Th2 type of immune response imbalance type inflammatory response,its immunopathologic characteristics are the large number of nasal mucosal tissue expression of Th2 type cytokines(IL-4,IL-5,IL-18)cell infiltration.With the development of science and technology,through the further study of AR pathogenesis,it is found that its mechanism has been extended from Th1/Th2 cell model to Tregs/Th17 model.At present,more attention is paid to allergic rhinitis in the respiratory system and the treatment is immunotherapy,but also mainly aimed at the respiratory ’system.Less research is on the intestinal system.Intestinal microbial formation has an effect on the immune system.The intestinal tryptophan affects the differentiation CD4+T naive helper cells into regulatory T cells(Tregs)and Th17 cells through AHR catabolism.The main metabolites of tryptophan bacteria were indole.Has some effect on the immune systemOBJECTIVE:Effects of intestinal metabolite indole-3-methanol(I3C)on allergic rhinitis.Methods:BALB/c mice were randomly divided into six groups:treatment group 1,treatment group 2,treatment group 3,solvent control group,model group,normal group.All groups except the normal group received ovalbumin and aluminum hydroxide adjuvant intraperitoneal injection and nasal drip,and the AR model was performed.The normal group received the same amount of normal saline intraperitoneal injection and nasal drip.After the treatment group 1,2 and 3 were successfully modeled,the I3C 0.8mg/day、0.4mg/day、0.2mg/day was mixed in olive oil and anhydrous ethanol for 1 week.The behaviors of mice were observed after 15 days of intraperitoneal injection and 7 days of nasal drip.ELISA detection of serum IL-10、IgE;flow detection of peripheral blood Tregs/Th17,anterior nasal histopathological sections to observe inflammatory cell content.After 1 week of gavage in treatment group 1,2,3 and solvent control group.Result:Behavioral scores,pathological results and IL-10、IgE、Tregs/Th 17 were not statistically different in normal group and treatment group.Results:1.Mouse symptom scoreCompared with the normal group,mice in the modeling group had more obvious symptoms such as sneezing,scratching nose and runny nose(p<0.05).However,there was no significant difference between treatment group and normal group(p>0.05)and no significant difference between solvent control group and normal group(p>0.05).2.Pathological changes of anterior nasal tissueThe anterior nasal tissue structure of the normal group was complete,the epithelial cells were arranged neatly,and a little inflammatory cell infiltration was seen.The modeling group and the control group saw the disorder of the anterior nasal tissue structure,hyperemia and hyperplasia in the stroma,and a large number of inflammatory cell hyperplasia.After I3C treatment group 1,treatment group 2,treatment group 3 mice and normal group mice nasal anterior tissue,there is a little inflammatory cell proliferation,compared with the solvent control group and modeling group significantly reduced.3.IL-10 and IgE levels across groups.The total level of IgE in the peripheral blood of the model group was significantly increased(p<0.05)compared with that in the normal group,while the IL-10 level in the peripheral blood of the model group was significantly decreased(p<0.05).I3C treatment group 1,2,3 compared with the solvent control group that was intervention after successful modeling,it was found that the total level of ige in peripheral blood of the treatment group decreased significantly(p<0.05),while the IL-10 level increased significantly(p<0.05).There was no significant difference between IL-10 and IgE in the peripheral blood of the model group and the solvent control group(p>0.05),and there was no significant difference between the treatment group(p>0.05).4.Tregs and Th17 changes between groupsThe model group Th17 significantly increased(p<0.05)and tregs significantly decreased(p<0.05)compared with the normal group;the treatment group 1,2 and 3 decreased significantly(p<0.05)and the Tregs significantly increased(p<0.05)compared with the control group;the Tregs and Th17 between the solvent control group and the model group were not statistically significant(p>0.05);the treatment group and the normal group were not statistically significant(P>0.05);There was no significant tregs and Th17 between treatment groups(p>0.05).Conclusion:I3C can improve the clinical symptoms and histopathology of Allergic Rhinitis mice and may play a role by increasing IL-10,reducing IgE,balance Tregs/Th17. |
