The Screening Of Anticancer Plants And Effective Ingredient's Separation And Purification

Malignant tumor is one of the main diseases for destroying people's health and life. Recently, operation and chemotherapy are reported as the main means of malignant tumor treatment. But clinical chemotherapy drugs not only damage the tumor cells but also impair the normal cells. Therefore.the treatment can't be carried out successfully. Traditional Chinese medicine for treatment of malignant tumor has a long history, Seek the compound with wonderful anti-tumor function and low side effect from the Chinese medicine is one important path to develop new anti-tumor drugs.Six Chinese herbs have been investigated .there names are as follows:(Crotalaria sessiliflora L.) (Rabdosia amethystoides Hara.) (Lobelia chinensis Lour.) (Amorphophallus Sinensis Belval) (Pinellia CordataN.E.Br. ) (Arisaema heterophyllum BL. ).Six Chinese herbs are extracted using the process of hot reflux with ethanol and concentrated into extractum. Through experiment in vitro using techniques of MTT, we found that the Lobelia chinensis Lour has good function of inhibiting the SGC-7901 tumor cells from growing.With the help of response surface analysis (RSA),the optimum condition for extraction was given: the Lobelia chinensis Lour 3.0g,ratio of solid and liquid 1:25,extracting time 4.6h,solvent(methanol) concentration 99%,extracting temperature 59.14℃.The study applied column chromatography to separate and purify and prepare the anti -tumor active compound of the Lobelia chinensis Lour. Weak polar or non-polar stuffing as SD₃₀₀, SD₃₀₁ and activated carbon (10-40um) are mainly chosed.. Based on the absorption amount and desorption ratio we finally chose SD3Oi. Different operation conditions such as sample concentration,temperature,flow rate, salt effect, proportion of diameter to height,sample pH are investigated for the influence on the stuffinging absorption .The optimum absorption condition was given: sample prepared with O.lmol/L NaCl solution,and keep sample pH at 7,absorbance at about 0.700, flow rate at 4ml/min, stuffing proportion of diameter to height 1:12.Choose different concentration of ethanol and acetone as elution solvent and study on the shape of peak to determine the elution flow rate...