Study On The Relationship Between Expression Of COX-2,Survivin And Microvessel Density In Gastric Carcinoma

Objective To investigate the expression of COX-2, Survivin in gastric carcinoma and their correlation with tumor microvessel density; To analyze their roles in tumor oncogenesis, progress and metastasis in gastric carcinoma.Methods Streptavidin-peroxidase-biotin immunohistochemistry technique was used to detect the expression of COX-2, Survivin and MVD (labled by CD34) in sixty gastric carcinoma specimens, fifty-two gastric mucosa membrane beside carcinoma specimens, twenty-five normal gastric mucosa membrane specimens. The relationship between the expression of COX-2, Survivin, MVD and the clinicopathological features in gastric carcinoma, the correlation among the expression of COX-2 , Survivin and MVD were analyzed statistically.Results (1) The positive expression rate of COX-2 in gastric carcinoma(70.00%) was significantly higher than in mucosa membrane beside carcinoma(13.46%) and in normal gastric mucosa membrane(0%) (P<0.01, P <0.01); It had a great relevancy to the lymph node metastasis and TNM stages(P <0.01, P <0.01), but no distinct difference was found in age, sex, tumor size, location, infiltration depth and differentiation(P > 0.05). (2) The positive expression rate of Survivin in gastric carcinoma(56.67%) was much higher than in mucosa membrane beside carcinoma(5.77 %) and in normal gastric mucosa membrane(0%) (P <0.01, P <0.01). The positive expression of Survivin in gastric carcinomas was significantly correlated with the infiltration depth and TNM stages(P <0.05, P <0.01), but incorrelated to differentiation, lymph node metastasis, age, sex, tumor size and location(P>0.05). (3) The positive expression of COX-2 and Survivin in sixty cases of gastric carcinoma tissues was closely associated with each other(r=0.308, P <0.05). (4) The positive expression of COX-2 and Survivin in sixty cases of gastric carcinoma tissues correlated with MVD, respectively(P <0.01, P <0.01), and MVD (55.46±17.86)in both COX-2(+) and Surviving)...