| Objective(1)Study the relationship between E2F1 expression and its biological behavior, as well as its relationship with the expression of Survivin and P53 in gastric carcinoma.(2) To restrain the proliferation of human gastric cancer line SGC7901, and induce it to apoptosis by using E2Fdecoy DNA assay.Methods(1)The expressions of E2F1, Survivin and P53 were detected by immunohistochemical method (SP) in 61 cases of gastric carcinoma, and compared with those in 20 cases of low-grade intraepithelial neoplasia(IN)tissues. The relations between them were analyzed.(2)The synthesized E2F decoy DNA was transfected into human gastric cancer line SGC7901 by liposome LipofectaminTM2000, change of cell morphology was observed by inverted phase contrasted microscope and cell livability was detected by MTT assay. Flow cytometry was applied to detect the change in apoptotic cell.Results(1)The positive rates of E2F1,Survivin and P53 were 75.4%, 85.2%, 68.9%, respectively, in gastric carcinoma, which were much higher than those(30.0%,40.0% and 15.0%) in low-grade intraepithelial neoplasia.(2)There was no significant difference in relationship between E2F1 gene expression in differentiation grade, invasion depth, TNM classfication, lymph node metastasis and distant metastasis (p>0.05). There was a significant difference in both Survivin and P53 gene expression in TNM classification and distant metastasis (p<0.05) and Survivin expression was significant different between non-and lymph node metastasis.(3)The expression of E2F1gene was positively correlated with that of Survivin and P53(rs1=0.519 ,p1<0.01; rs2=0.602 ,p2<0.01).(4) After human gastric cancer line SGC7901 was transfected with E2F decoy DNA,we observed the cell morphology by sharing time, and found that the size of the transfected cell was becoming smaller as time prolonged, the cytoplasma was becoming vesicatorious, and the nuclear membrane was becoming wizened, at 48h or so after transfection, the apoptotic body could be found.(5) The result of cell livability by MTT assay showed that in the group using E2F decoy DNA, the livability of cell SGC7901 was low, which was significantly different with the control decoy DNA group and the blank control group (p<0.05),there was no significantly different between the control decoy DNA group and the blank control group (p>0.05).The cell livability of E2F decoy DNA group were 65.3%,48.3%and 48.5%, respectively ,at 24h, 48h and 72h after transfetion.(6) The apoptosis rate and mortality of cell SGC7901 were 14.75% and 2.43%, respectively at 24h, and 22.15% and 20.58% at 48h, after transfection by E2F decoy DNA, which had significant difference with the control decoy DNA group and the blank control group (p<0.05).Conclusion(1)E2F1, Survivin and P53 have a coordinated action in the oncogenesis and progression of gastric carcinoma.(2)E2F1 may play a role in the early phase of gastric carcinoma, while Survivin and P53 may work in the late phase of that.(3)By tansfectd with E2Fdecoy DNA,human gastric cancer line SGC7901 can be induced to apoptosis.(4)The transcription factor"decoy"strategy may be a new gene therapy approach to gastric cancer by inducing the deletion of gene function.
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