Determination Of The Main Composition In The Herb Of Magnolia Officinalis For Eliminating Oxygen Radicals And The Anti-aging Effect Of Honokiol

The extraction of Magnolia Officinalis possesses the antioxidantive function, but it is not clear which compositions play a main role and whether the compositions possess the antiaging effect. The relational experiments are done to solve the problems.(1) The elimination action of magnolol and honokiol for hydroxyl radical and hydrogen peroxide are determined with fluorescence spectrophotometry, comparing their half elimination ratio with those of Magnolia Officinals, the main efficient composition scavenging oxygen radicals can be determined.(2) The inhibitor effect of honokiol on lipid peroxide is determined by measuring the malondehyde (MDA) of mice tissue (myocardium, brain, liver) in vitro and in Cys-Fe²⁺ system.(3) Giving mice different dose of honokiol, their antistress effects can be evaluated by measuring the swimming time and the survival time of hypoxia under the ordinary atmosphere.(4) The subacute senile mice model is established by injecting D-galactode 0.30g/(kg·d) for 42 days. All mice are divided into five groups (control group, model group, VE positive control group, high dose group, low dose group). The anti-aging effect of honokiol can be evaluated by measuring the activity of SOD, GSH-Px, CAT and the content of MDA existing in serum, myocardium, brain and liver.The result show the half elimination ratio of magnolol and honkiol are 0.081mg/L and 0.091 mg/L for hydroxyl radical, and 2.357mg/L and 2.772 mg/L for hydrogen peroxide, respectively. The content of magnolol and honokiol in the herb of magnolia officinalis is only 4.64%, however, their elimination action to two kinds of oxygen radicals is up to 56.70% and 54.91% those of magnolia officinalis, so we confirm magnolol and honokiol are the main efficient composition eliminating oxygen radical in the herb of magnolia officinalis. The result of anti-hypoxia experiment show two dose (0.002g/(kg·d), 0.01 g/(kg·d)) of honokiol can obviously prolong the survival time of mice, the prolonging ratio are 16.58% and 35.66% respectively. In the experiment of antifatigue, the positive effect of honokiol is also shown, the expanding ratio of swimming time are 31.63% and 85.98%.