| Objective: Various diseases of central nervous system (CNS) are now treated with Gamma Knife (γ-knife) radiosurgery. While more and more patients choose γ-knife as their treatment modality, some of them suffered form its serious complication—CNS radiation injury. Since the mechanism of CNS radiation injury still remains unclear, more researchers are focusing on this area. They find the expression of GFAP and VEGF altered in CNS after irradiation. We made the present experiment to study the time-related expression of GFAP and VEGF during the subacute phase in the normal rat brain after γ-knife irradiation, discuss the possible relationship.between active astrocytosis, strong expression of VEGF and CNS radiation injury.Methods: Thirty adult female Wistar rats were randomly divided into six groups, five rats in each group. One group served as sham irradiation group while the other five groups underwent γ-knife irradiation. Rat brains were scanned by Toshiba 1.5T MRI and images were transported to γ-plan 5.31 version dosage planning system. The right caudate-putamen nucleus was selected as the target of irradiation. A 4-mm collimator was used to transmit 50Gy dosage to the target region. Rats were killed at 1, 2, 4, 8, 12weeks after irradiation. Serial brain sections were processed with the immunohistochemical avidin-biotin complex technique, using anti-GFAP and anti-VEGF as the primary antibody.Results: The number of GFAP positive cells (astrocytes) in the target...
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