Effect Of Compound Ion Salt On Left Ventricular Hypertrophy And Other Related Factors Of Two Kidney-one Clamp Renovascular Hypertensive Rats

Objective The purpose of this study was to investigate the effect of compound ion salt, Valsartan and cyclosporineA on myocardial hypertrophy and other related factors, using two kidney-one clamp renovascular hypertensive rats, trying to discuss the effect of compound ion salt on the left ventricular remodeling process caused by hypertension. Methods The rat model of secondary hypertension was induced by two kidney-one clamp method. Rats with systolic blood pressure more than 20 mmHg assessed by non-invasive tail method 4 weeks later, were considered the successful model and were randomly divided into following groups for 12 weeks: compound ion salt group, Valsartan group, compound ion salt plus Valsartan group, cyclosporineA group and control group. 10 rats of same age were operated to expose renal artery without clamping were used as sham operation group. 12 weeks later, rats were anesthetized for invasive hemodymanic measurement, then the rats were sacrificed. Some of hearts were removed and prepared for histological analysis and others were quickly freezed by liquid nitrogen and stored in -80 °C for further assay. Tissue slide was stained by picrosirius red, a collagen specific method, and the content of collagen was expressed as collagen volume fraction and ratio of type Ⅰ to type Ⅲ collagen by image analysis software. The mRNA expression of c-fos was quantitatively analyzed by in situ hybridization. The mRNA level of Calcineurin was assessed by reverse transcriptase PCR. The level of endothelin, angiotensin Ⅱ, aldosterone and atrial natriuretic peptide in left ventricles were determined by radioimmunoassay. Nitric Oxide level in theventricle was assessed by biochemical method.Results 1. The intake of compound ion salt could ameliorate left ventricular remodeling process caused by hypertension. The ratio of left ventricular weight to tibial length (Lvw/TL) (26.33±3.95 g/m vs 34.15±6.55g/m, PO.01), collagen volume fraction(15.73%±1.40% vs 18.60%±2.20%, PO.05), atrial natriuretic peptide(72.71±34.98 ng/g vs 231.89±32.51 ng/g, PO.01) and Calcineurin mRNA expressing level(0.82±0.10 gray level vs 0.96±0.12 gray level, PO.Ol) were all lower than those in common salt group.2.Valsartan had beneficial effects on left ventricular remodeling process. The Lvw/TL(24.82±2.68 g/m vs 34.15±6.55 g/m, P<0.Q\ ), collagen volume fraction(8.16%±0.80% vs 18.60%±2.20%, P<0.01), atrial natriuretic peptide (66.63±31.34 ng/g vs 231.89±32.51 ng/g, PO.01) and Calcineurin mRNA expressing level (0.69±0.08 gray level vs 0.96±0.12 gray level, /><0.01) were all lower than those in common salt group.3. CsA had no obviously ameliorating effects on ventricular remodeling process. Between CsA group and common salt group , there were no differences in collagen volume fraction(16.47%±2.06% vs 18.60%±2.20%, P>0.05) and perivessel collagen area(104.12%±23.76% vs 114.32%±18.10%,4. The effects of compound ion salt on ventricular remodeling process were less potent than those of Valsartan group. The collagen volume fraction(15.73%±1.40% vs 8.16%±0.80%, /><0.01) and Calcineurin mRNA expressing level (0.82±0.10 gray level vs 0.69±0.08 gray level. PO.01) were all higher than those in Valsartan group.5. Compound ion salt plus Valsartan had potentially beneficial...