The Establishment Of Pharmacological Evaluating Methods On Improving Sleep Effect

A pharmacological evaluating experiment system on improving sleep effect was established by connecting behavior observation method with electrophysiological technique in the thesis. It is made of two parts: one is experiment method of sleeping time in Mice with Pentobarbital Na; the other is recording and analyzing the electroencephalogram (EEG) of sleeping rats. The first method can only provide some preliminary central inhibition information. Improving sleeping effect will be confirmed by the latter method. We found the above methods are affected by many factors. To make it more accurate and credible, correlate factors were studied.Firstly, regarding total sleeping time as index, effects of weight , sex, dosage of Pentobarbital Na and experimental time on sleeping time with Pentobarbital Na in mice were investigated. The results indicate that the accuracy of the result was affected by different dosage. The coefficient of variation of dosage above threshold is lower than that of threshold dosage. It is better to conduct this experiment with dosage of 55mg/kg. There is no significant difference on the result between male and female, but the coefficient of variation of female is bigger than that of male distinctly. It is necessary to choose male mice as experimental animals. The weight of mice may affect the result. With the increasing of weight, sleeping time with Pentobarbital Na in mice showed a trend of decrease distinctly. If the tested drug is synthetic (single dose),it is better to choose mice weighting 20~ 22g,because their sleeping time is moderate and the experimental error is little. If the tested drug is herb, it is better to choose the mice weighting 18~20g. After the last administering the drug, it is...