Effect Of NDGA, A General Lipoxygenase Inhibitor, On The Proliferation And Apoptosis In HepG2 Cells In Vitro

objective: To investigate the effects of lipoxygenase inhibitorson the proliferation and apoptosis of HepG2 cells and its relevant mechanism.Methods: human hepatoma cells line HepG2 trained outside the body,the effects of lipoxygenase inhibitors (nordihydroguaiaretic acid; NDGA ) on the growth and survival of human hepatoma cells line HepG2 were determined with microculture tetrazolium assay (MTT), growth curve and colony-forming assay and apoptosis and cells cycle analysis was determined using HE staining and flow cytometry. Measured the protein expression of Caspase~3, PCNA with immunohistochemistry. Western blot analysis were employed to observe the effec to NDGA on the expression of wtp53 and c-erbB-2 protein.Results: NDGA had marked suppressive effect on the growth of HepG2cells line.The cells were treated with varies concentrations of NDGA(50, 100, 150, 200)μmol/l for up to 72 hr Cells proliferation was measured using MTT. The inhibitory rates of the cells were (27.34± 5. 95)%, (41. 76 + 7. 47)%, (57. 08 + 4. 36)% and (69. 17 + 4. 42)% respectively in 72 hours. NDGA had a remarkalbe cytotoxic effect to HepG2 with IC50 of almost 109. 13μmol/L. Colony-formating rate of HepG2 cells were (21.63 + 0.70)%, (17. 33 + 0. 96)%, (12.53 + 0.45)%, (7. 97 + 0. 92)% respectively, which were much lower than that of control group (31. 70 + 0. 62)%, (P<0. 01). At the same time, Flowcytometry revealed NDGA could induce the apoptosis of HepG2 with the cells cycle arrested on G, phase. NDGA could stimulated apoptosis in liver tumor HepG2 cells line, the apoptotic rate was (3.00...