Study On The Expression And Significance Of CD105,MMP-2 And MMP-9 In Giant Cell Tumor Of Bone

Objective: To study the expression levels of CD105, MMP-2 and MMP-9 in giant cell tumor of bone and detect the correlation between their expression levels and the histological grade, radiological stage, recurrence state of the tumor. To intend to find possible prognostic indications of the aggressive behavior of the underlying pathology . Methods: The expression of CD105, MMP-2 and MMP-9 in 38 cases of giant cell tumor of bone was studied by using SP immunohisto-chemical method. The relationship between the expression levels and clinical-pathological features was also analyzed. Results: (1) The average MVD marked by CD105 in giant cell tumor of bone was 9.70±2.34. No significant difference was found among pathology grade Ⅰ,Ⅱand Ⅲcases (P<0.05), while significant difference was found between radiological stage Ⅲ( aggressive type ) and stage Ⅰ(quiescent type) cases (P<0.05). Recurrent cases had higher MVD than cases without recurrence(P<0.01). MVD may be associated with tumor's radiological stage and its recurrence. (2) Overexpression of MMP-2 was found in the tumor. Positive rate of MMP-2 was 47.37%. No distinct difference of the expression levels of MMP-2 was found among cases of different histograde and radiological stage (P>0.05). Recurrent cases had higher positive rates of MMP-2 than cases without recurrence (P<0.05). Overexpression of MMP-2 may be associated with the recurrence . (3) Overexpression of MMP-9 was also found in giant cell tumor of bone. Positive rate of MMP-9 was 60.53%. No distinct difference of the expression levels of MMP-9 was found among cases of different histological grade (P>0.05). Tumors of radiological stage Ⅱand Ⅲ(aggressive type and active type) had higher positive rates than stage Ⅰ(quiescent type) cases (P<0.01), and recurrent cases had higher positive rates than cases without recurrence (P<0.05). Overexpression of MMP-9 may be associated with the recurrence of the tumor .(4) MMP-2 positive cases had higher MVD than MMP-2 negative cases. MMP-9 positive cases had higher MVD than MMP-9 negative cases(P<0.01). Conclusions: MVD marked by CD105 and overexpression of MMP-2, MMP-9 can be used as possible prognostic indications of tumor's recurrence state. Angiogenesis in tumor might be promoted by MMP-2 and MMP-9.