Expression Of CD34,CD105,MMP-9 In Spinal Giant Cell Tumor And Their Biological Significance

Background and Objective:Giant cell tumor of bone(GCT) derived from mesodermic cell, consists mostly of multinucleated giant cells and ground substance cells. In addition, it is generally accepted that ground substance cells is the main part of GCT. The incidence of GCT of bone is about 10-15% in China,high than 5 -8% in western countries. Surgery is the most important treatmeng for GCT of bone, which is an invasive potentially malignant tumor. However, the postoperative recurrence is still very high. It is hard to prognoses this disease according to the current standard pathohistological classification. For example, some patients who were considered with benign tumor using this classification usually develop local recurrence or even lung matastasis .In this case,some schllars called GCT an "unpredictive" tumor. So how to identify a tumor specific maker which is important in the design of an interventional procedure, how to precisely predict the biological behavior of the tumor and how to select the proper therpy to reduce the recuurence are key points concerned by clinician.Evidence showed neogenetic vascular in tumors has a closed relationship with biological development of the tumor. And some documents have reported that MMP-9 was one of the close factors which caused the generation of the neogenetic vascular,and microvascular density(MVD) is one of the important index which can well refleck the intensity of the tumor-related neogenetic vascular. So the objective of the research is to construct tissue chip of spinal giant cell tumor with normal tissure near tumor as control, and detect the expression of CD34,CD104,MMP-9 in the tissue array of spinal giant cell tumor , then to detect the correlation of the exppresion of proteins and the biological process of this tumor,the clinical and pathological data by statistic analyses.Methods:A total of 45 cases of spinal giant cell tumor underwent surgical treatment in Changzheng Hospital . the first affiliated hospital of WenZhou medical university and the secoseond affiliated hospital of WenZhou medical university from Jan. 1998 to Jan. 2007 with complete clinical data and partly follow up data, as well as ten normal tissure near tumor was included in this study .A tissue microarray was built by a tissue microarrayer. Tissue chips of spinal giant cell tumor were obtained from serial sections of tissue microarray. The expression of CD34,CD104,MMP-9 were detected on the tissue chips by immunohistochemistry to detect the abnormal expression of the proteins. The Stata7.0 statistical software was used in analyses on different exppresion between tumor tissue and normal tissue, relationship between each gene expression and the clinical and pathological data.Results:1. The tissue microarray of spinal giant cell tumor was built and tissue chips were produced from it.2.The positive expression of MMP-9, locating in the cytolymph with ,shows dark yellow or brown. The expressions of MMP-9 between spinal giant cell tumor and non-tumor tissue have a remarked difference (P<0.01).The neogenetic vascular endothelial cell(VEC) marked by CD105 protein locates in the margin in tumor,none expression in the normal tissue,and colores cytomembrane and cytolymph with yellow or brown,dark yellow or brown. The expressions of MVD marked by CD105 and CD34 between spinal giant cell tumor and non-tumor tissue have a remarked difference (P<0.01). 3.MVD in positive expression team of MMP-9 is more remarkable than which in negative expression team(P<0.01) .Both expression of CD105-MVD and CD34-MVD have a positive relation with expression of MMP-9 in spinal giant cell tumor(P<0.01).Compared with CD105-MVD,the expression of CD105-MVD has closer relation with the expression of MMP-9( r=0.5290,r=0.4365 P<0.05 )= 4.The expressions of CD105-MVD and MMP-9 between differenet pathological grade in spinal giant cell tumor have a remarked difference (P<0.01) ,but the expressions of CD34- MVD and MMP-9 between differenet pathological grade in spinal giant cell tumor have no difference (P>0.05).The expression of CD34 had no relation with patient's age,sex,soft involvement and tuomr location in spinal giant cell tumor (P>0.01). The expression of MMP-9,CD105-MVD had a possitive relation with pathological grade of the tumor (ρ1 = 0.5100, P<0.05; p2 = 0.6681, P<0.05 ), but CD34-MVD had no relation with pathological grade of the tumor in spinal giant cell tumor ( p3 = 0.2003, P>0.05 ) .Conclusion:1.The applying of the technique of tissue microarray in the study of spinal GCT was well preserved and stable. 2.MMP-9, CD105 and CD34 play important role in malignant pregression of tumors ; MMP-9 , CD105 and CD34 have no relation with clinicopathological features like patient's age,sex,soft involvement and tuomr location. CD105 is more specific than CD34 to reflect the proliferation of spinal giant cell tumor.