Novel Treatment Methods And Characteristic Evaluation Of Xenogenic Bone

Bone defect is one of the most common diseases in osteology clinic. Autogeneic bone transplantation, xenogeneic bone transplantation as well as vascular anatomizes bone transplantation are the traditional strategies used today, and xenogeneic bone has an advantage of largely supplied.In recent years, because of the enormous development and intersection between cytobiology and biomaterial engineering science, tissue engineering has forged ahead rapidly as a novel frontier science. Developed gene and tissue engineering technology give a promising method to deal with bone defect.Bone tissue engineering is a hot spot among the researches nowadays. Its main content includes three aspects of study: scaffolding materials which cells can be seeded on; seed cells characteristics; growth factors related with accelerating bone reconstruction. Of which, the first one is the core for the tissue engineering and the key if it can be for the clinical application. In this study, supercritical CO₂ fluid extraction technology was employed to delipidate pig cancellous bone. Low temperature bating enzyme; hydrogen peroxide maceration and ultrasonic were used to remove the proteins from the samples to prepare the new extracellular matrix material. We observed the material by thermogravimetric analyses; scanning electron microscopy; X-ray diffraction analyses and used the material to carry osteoblast came from rabbit embryo's bone tissue, carried out a series of experimental studies. The results showed that the new extracellular matrix material maintain the natural tri-dimensional porous structure in the cancellous bone, have high porosity, and well biocompatibility, is a promising tissue engineering scaffold materials for bone repairs.