The Action Of Nitric Oxide On Spinal Cord Injury Of Adult Rats After Transplantation Of The Microencapsulated Rabbit Nervous Tissue

Objective To observe the influence of the NOS expression on the spinal cord half transection injury of adult rat after transplantation of the microencapsulated rabbit sciatic nerve tissue, Study the mechanism that the transplantation with microencapsulated different race nervous tissue/cells treats the spinal cord injury(SCI). Methods A total of 130 adult Sprague-Dawley(SD) rats were randomly divided into four groups: Group A,B,C and D. 10 rats of Group D were normal control. 40 animals in each group of A, Band C were performed a lateral hemisection of spinal cord at right T10 level, then with nerve's tissue/cells(Group B) and with microencapsulated nerve's tissue/cells(Group A) were implanted into the injured area respectively. Group C were implanted nothing. 3, 7 and 14 days post-injury, 10 rats of each group in A, B and C as well as the normal rats were anesthetized and perfused transcardially with 4% paraformaldehyde(the pH7.4). Take out 8mm sections of the thoracic cord including the injured site and the spinal cord of the normal rats at corresponding level. frozen section(the coronal, 25 u m ). Then dealed with Nissl staining and NADPH-d histochemistry. Analysis with the image analysis system. Results After SCI the number of the neurons reduced and the Nissl substance lost or dissolved. On the 14th day the number of the Nissl stained neurons rose remarkably and their volume increased. These changes were seen most evidently in Group A(P<0.05). NOS positive reaction showed the blue black, which in Group A and B were superior to that in Group C(P<0.05) on the 3rd day post-injury. The expression of NOS in Group A was higher than Group B(P<0.01) on the 7th day. on the 14th day, cell number with NOS positive reaction Group B heightened rapidly, and even beyonded Group A. however, Group A showed an steady increasing tendency. Conclusion The xenotransplantation of micro-encapsulated sciatic nerve's tissue/cells can induce the NOS expression increment in the earlier period and inhibit NO over dose creations anaphase, can improve the functional recovery, being advantageous to the repair and the regeneration of the injury spinal cord.