A Comparative Study Of Exopolysaccharides Produced By Planktonic And Attached Actinomyces Viscosus

To elucidate the biofilm structure of Actinomyces viscosus and the spatial distribution of exopolysaccharides in it. To comparatively evaluate the exopolysaccharides production of adhesive Actinomyces viscosus and planktonic ones and further investigates the reasons for these differences.The Actinomyces viscosus biofilm was made by allowing bacterial to attach to the cover glass surface. The biofilm structure at 24 hours and exopolysaccharides distribution were visualized with fluorescent staining Fluorescein , BODIPY and Calcofluor respectively by confocal laser scanning microscopy (CLSM). The anthtone method was applied to measure the production of soluble and insoluble exopolysaccharides by adhesive Actinomyces viscosus and planktonic ones.Actinomyces viscosus can attach to glass surface and form structural biofilm, which Bacteria were embedded within the EPS glycocalyx polymers and characteristic microcolonies and channels were taken shape.Bacteria were sparse in the substratum area but crowd in the center. Adhesive Actinomyces viscosus produced more insoluble exopolysaccharides than planktonic ones under varied sugar concentrations(P<0.05).Actinomyces viscosus in Biofilm can produce more insolubleexopolysaccharides, which plays an important role in the procession of biofilm formation. The phenotypic change of selfsame bacteria may visualize new biochemical property in specific growth environmental conditions of the biofilm.